Apr 22, 2020

Tgfbr2 in dental pulp cells guides sensory innervation in developing teeth

BioRxiv : the Preprint Server for Biology
C. BarkleySarah Beth Peters


Transforming growth factor {beta} (TGF{beta}) plays an important role in tooth morphogenesis and mineralization. Our laboratory established a mouse model in which Tgfbr2 was conditionally deleted in the dental pulp (DP) mesenchyme using an Osterix promoter-driven Cre recombinase (Tgfbr2cko). These mice survived postnatally, but had significant defects in bones and teeth, including reduced mineralization and short roots. H&E staining revealed reduced axon-like structures in the mutant mice. Reporter imaging demonstrated that Osterix-Cre activity was localized within the tooth and was active in the DP and derivatives, but not in neurons. Previous research has established that paracrine signals from the dental pulp attract trigeminal axons toward and into the tooth during postnatal development, yet very little is known about the signals that regulate tooth innervation. Immunofluorescent staining for a neuronal marker, {beta}3 tubulin, was performed on serial cryosections from control and mutant molars at two different postnatal stages. Confocal imaging and pixel quantification of {beta}3 tubulin demonstrated reduced innervation in P7 and P24 Tgfbr2cko first molars compared to controls, indicating that the signals necessary to prom...Continue Reading

  • References
  • Citations


  • We're still populating references for this paper, please check back later.
  • References
  • Citations


  • This paper may not have been cited yet.

Mentioned in this Paper

MDM2 protein, human
MDM2 gene
Bounded by
Men Who Have Sex With Men
Molecular Docking Simulation
Docking -molecular Interaction
Cancer Treatment

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.