Maspin retards cell detachment via a novel interaction with the urokinase-type plasminogen activator/urokinase-type plasminogen activator receptor system

Cancer Research
Shuping YinShijie Sheng

Abstract

It is well documented that tumor suppressive maspin inhibits tumor cell invasion and extracellular matrix remodeling. Maspin is a cytosolic, cell surface-associated, and secreted protein in the serine protease inhibitor superfamily. Although several molecules have been identified as candidate intracellular maspin targets, the extracellular maspin target(s) remains elusive. Although maspin does not directly inhibit urokinase-type plasminogen activator (uPA) activity, we have shown evidence that maspin may block the pericellular proteolysis mediated by cell surface-associated uPA. In the current study, maspin significantly inhibited the Ca2+ reduction-induced detachment of DU145 cells. This maspin effect was associated with increased and sustained levels of mature focal adhesion contacts (FAC). We noted that maspin (a) colocalized with uPA and uPA receptor (uPAR), (b) enhanced the interaction between uPAR and low-density lipoprotein receptor related protein, and (c) induced rapid internalization of uPA and uPAR. The maspin effects on surface-associated uPA and uPAR required the interaction between uPA and uPAR. Further biochemical and biophysical analyses revealed that maspin specifically bound to pro-uPA with a deduced K(d) of 2...Continue Reading

References

Jan 1, 1991·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Y P KeepersH M Pinedo
Apr 1, 1970·The Journal of Trauma·D S BradfordH L Nossel
Jan 1, 1994·Cold Spring Harbor Symposia on Quantitative Biology·R SagerP Pemberton
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·S S SchneiderG A Silverman
Apr 1, 1994·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·L BastholmM H Nielsen
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·S ShengR Sager
Feb 12, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D TsatasG E Rice
Feb 2, 2000·Nature Medicine·M ZhangN Bouck
May 31, 2002·Oncogene·Ning JiangShijie Sheng
Mar 5, 2003·The Journal of Cell Biology·Ralf-Peter CzekayDavid J Loskutoff
Mar 15, 2003·Nature Reviews. Cancer·John D Hood, David A Cheresh
May 2, 2003·Nature Reviews. Cancer·Peter Friedl, Katarina Wolf
Jun 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michael L CherShijie Sheng
Sep 26, 2003·Cancer Biology & Therapy·Valerie A Odero-MarahMary J C Hendrix
Dec 31, 2003·The Journal of Biological Chemistry·Ana María SalicioniSteven L Gonias
Mar 5, 2004·Cancer Research·Jiayou LiuShijie Sheng
Apr 28, 2004·Current Opinion in Genetics & Development·David D Schlaepfer, Satyajit K Mitra
Sep 9, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Shijie Sheng
May 18, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Xiaoying YinCarol G Shores

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Citations

Feb 14, 2008·Cell Research·Shuomin ZhuYin-Yuan Mo
Feb 4, 2010·The Journal of Biological Chemistry·Sonia S Y TeohPhillip I Bird
Aug 18, 2010·The Journal of Biological Chemistry·Li Qin, Ming Zhang
Sep 15, 2010·The Journal of Biological Chemistry·Lorna RavenhillRosemary Bass
Nov 1, 2006·Anti-cancer Drugs·Shinichi NozakiEtsuhide Yamamoto
Mar 19, 2013·Clinical and Translational Medicine·Rossana BerardiStefano Cascinu
Nov 13, 2012·Critical Reviews in Eukaryotic Gene Expression·Alexander KaplunShijie Sheng
Nov 21, 2012·Nutrition and Cancer·Teresa García-GascaAlejandro Blanco-Labra
May 27, 2014·International Journal of Molecular Sciences·Mohini SinghSheila K Singh
Apr 21, 2010·Expert Opinion on Investigational Drugs·Ralf HildenbrandPhilipp Stroebel
Sep 4, 2015·International Journal of Molecular Medicine·Jun ZhouFeng Guo
Mar 23, 2010·Journal of Theoretical Biology·Hermann B FrieboesVittorio Cristini
Mar 28, 2008·Urologic Oncology·Devon C HallSusan S Padalecki
Jan 2, 2008·Biochimica Et Biophysica Acta·A M JoshuaJ A Squire
May 4, 2007·NeuroImage·Hermann B FrieboesVittorio Cristini
Oct 27, 2015·Journal of Theoretical Biology·M A Al-MamunR Bass
May 29, 2009·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Kunio YoshizawaEtsuhide Yamamoto
Apr 27, 2007·Journal of Cellular Physiology·Xiaohua LiShijie Sheng
Mar 7, 2017·Journal of Cellular Biochemistry·Margarida M BernardoShijie Sheng
Sep 27, 2006·Journal of Cellular Physiology·Caleb M BaileyMary J C Hendrix
Sep 3, 2010·Nonlinearity·J S LowengrubV Cristini
May 19, 2007·The Journal of Biological Chemistry·Maher Al-AyyoubiPeter G W Gettins
Nov 4, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Brigitte GouletJohn D Lewis
Oct 17, 2017·Oncotarget·Hua-Chuan Zheng, Bao-Cheng Gong
Apr 29, 2009·Journal of Clinical Pathology·S M KhoshnawI O Ellis
Feb 16, 2007·Cell Motility and the Cytoskeleton·Heidi Y ShiMing Zhang
Oct 21, 2015·Journal of Biomedical Science·Zhi-Yuan ChenYing-Ming Liou
Nov 11, 2014·Biological Chemistry·Yolanda Fortenberry
Nov 7, 2014·Oncotarget·Sijana H DzinicShijie Sheng
Sep 20, 2006·Cancer Research·Xiaohua LiShijie Sheng
Jul 26, 2015·Cancer Research·M Margarida BernardoShijie Sheng
Sep 27, 2014·Molecular Cancer Research : MCR·Thomas M BodenstineMary J C Hendrix

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