Mass Cytometry Studies of Patients With Autoimmune Endocrine Diseases Reveal Distinct Disease-Specific Alterations in Immune Cell Subsets.

Frontiers in Immunology
Louise MagnussonRosaura Casas

Abstract

Although there is evidence that autoimmune diseases share similar immunogenetic mechanisms, studies comparing peripheral CD45+ cells from patients with autoimmune endocrine diseases in parallel are limited. In this study, we applied high-dimensional single-cell mass cytometry to phenotypically characterize PBMC from patients with new-onset (N-T1D) and long-standing type 1 diabetes, Hashimoto's thyroiditis (HT), Graves' disease and autoimmune Addison's disease (AD), as well as healthy controls. The frequency of CD20loCD27hiCD38hiHLA-DRint plasmablasts, CD86+CD14loCD16+ non-classical monocytes and two subsets of CD56dimHLA-DR+IFN-γ+ NK cells were increased in patients with HT. Subsets of CD56dimCD69+HLA-DR- NK cells and CD8+ TEMRA cells, both expressing IFN-γ, were expanded and reduced, respectively, in the N-T1D group. In addition, patients with AD were characterized by an increased percentage of central memory CD8+ T cells that expressed CCR4, GATA3, and IL-2. We demonstrate that patients with N-T1D, HT, and AD had altered frequencies of distinct subsets within antigen-presenting and cytotoxic cell lineages. Previously unreported alterations of specific cell subsets were identified in samples from patients with HT and AD. Our s...Continue Reading

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Citations

Sep 2, 2020·Scandinavian Journal of Immunology·André SulenBergithe E Oftedal
Jul 4, 2020·Current Opinion in Endocrinology, Diabetes, and Obesity·Adeeb H Rahman, Dirk Homann
Aug 9, 2020·Frontiers in Endocrinology·Daniela GalloLorenzo Mortara
Mar 9, 2021·Frontiers in Immunology·Elena GianchecchiAlessandra Fierabracci

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Methods Mentioned

BETA
biopsies
hormone replacement
density-gradient centrifugation
FCS
PMA

Software Mentioned

Cytobank
Cytosplore
Cytofast
GraphPad Prism
+ HSNE
HSNE
CyTOF

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