Mass-producible microporous silicon membranes for specific leukocyte subset isolation, immunophenotyping, and personalized immunomodulatory drug screening in vitro

Lab on a Chip
Andrew StephensKatsuo Kurabayashi

Abstract

Widespread commercial and clinical adaptation of biomedical microfluidic technology has been limited in large part due to the lack of mass producibility of polydimethylsiloxane (PDMS) and glass-based devices commonly as reported in the literature. Here, we present a batch-fabricated, robust, and mass-producible immunophenotyping microfluidic device using silicon micromachining processes. Our Si and glass-based microfluidic device, named the silicon microfluidic immunophenotyping assay (SiMIPA), consists of a highly porous (∼40%) silicon membrane that can selectively separate microparticles below a certain size threshold. The device is capable of isolating and stimulating specific leukocyte populations, and allows for measuring their secretion of cell signaling proteins by means of a no-wash homogeneous chemiluminescence-based immunoassay. The high manufacturing throughput (∼170 devices per wafer) makes a large quantity of SiMIPA chips readily available for clinically relevant applications, which normally require large dataset acquisitions for statistical accuracy. With 30 SiMIPA chips, we performed in vitro immunomodulatory drug screening on isolated leukocyte subsets, yielding 5 data points at 6 drug concentrations. Furthermor...Continue Reading

References

Mar 1, 1995·Clinical Pharmacology and Therapeutics·W J JuskoQ A Mekki
Feb 15, 2008·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Richard HoldichSergey Zhdanov
Nov 18, 2011·Lab on a Chip·Weiqiang ChenJianping Fu
Feb 10, 2012·Lab on a Chip·Erwin BerthierDavid Beebe
Feb 22, 2012·Nature Reviews. Immunology·Holden T MaeckerRobert Nussenblatt
Mar 14, 2014·Nature·Eric K SackmannDavid J Beebe
Jun 24, 2014·Trends in Biotechnology·Lisa R Volpatti, Ali K Yetisen
Aug 30, 2014·World Journal of Gastroenterology : WJG·Jun-Jun JiaShu-Sen Zheng
Jun 4, 2015·Clinical Pharmacokinetics·Christine E Staatz, Susan E Tett
Oct 8, 2015·Therapeutic Drug Monitoring·Brunet MercèLópez-Hoyos Marcos
Jan 24, 2016·Clinical Biochemistry·O Millán, M Brunet
Jun 11, 2016·Cytometry. Part B, Clinical Cytometry·C Wyatt ShieldsGabriel P López

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme
flow cytometry
ELISA
fluorescence activated cell sorting
FACS
AlphaLISA
chip
chips
biopsy
sandwich immunoassay

Software Mentioned

COMSOL
ImageJ
LabView
SiMIPA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.