Mass spectrometric analysis of the immunodominant glycan epitope of Echinococcus granulosus antigen Ag5.

International Journal for Parasitology
Katharina PaschingerIain B H Wilson

Abstract

In previous work we showed that Ag5, a major diagnostic antigen from the metacestode of Echinococcus granulosus, possesses a dominant sugar epitope that upon removal results in abolition of most of the antigen immunoreactivity with patient sera. Analysis of this glycan modification has now been performed by western blotting and mass spectrometry. Reactivity to both a specific monoclonal antibody (TEPC15) and human C-reactive protein as well as the presence of a modification of 165 mass units, as detected by mass spectrometry of both glycopeptides and released N-glycans, indicated that the immunodominant sugar epitope of the Ag5 38kDa subunit is a biantennary structure modified by phosphorylcholine. We believe this is the first time that such a modification has been proven in cestodes and provides the structural basis for understanding the antigenicity of this major E. granulosus component.

References

Sep 1, 1987·Molecular and Biochemical Parasitology·J C Shepherd, D P McManus
Dec 1, 1989·Molecular and Biochemical Parasitology·M W LightowlersM D Rickard
Dec 1, 2001·The Journal of Biological Chemistry·Andreas J HülsmeierPeter Köhler
Oct 3, 2002·The Biochemical Journal·Carmen LorenzoGualberto González-Sapienza
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Mar 28, 2007·Enfermedades infecciosas y microbiología clínica·David CarmenaElena Eraso
Jan 7, 2009·Analytical Biochemistry·Thomas IskratschIain B H Wilson
Oct 11, 2011·Glycobiology·Katharina PaschingerIain B H Wilson

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Citations

Sep 17, 2013·Applied Microbiology and Biotechnology·Martin DragositsDubravko Rendic
Dec 10, 2015·Analytical and Bioanalytical Chemistry·Iain B H Wilson, Katharina Paschinger
Oct 2, 2015·Parasite Immunology·A DíazA M Ferreira
Oct 22, 2016·Biochimica Et Biophysica Acta. General Subjects·Carmen Jiménez-CastellsKatharina Paschinger
Apr 27, 2017·Journal of Proteomics·Karina M MonteiroHenrique B Ferreira
Dec 30, 2015·The American Journal of Tropical Medicine and Hygiene·Jianrong YeHong Zheng
Oct 31, 2015·Molecular & Cellular Proteomics : MCP·Alba HykollariKatharina Paschinger
Aug 15, 2018·Molecular & Cellular Proteomics : MCP·Alba HykollariKatharina Paschinger
May 7, 2019·Parasitology·Carolina De Marco VeríssimoAlessandra L Morassutti
Jul 7, 2019·Glycoconjugate Journal·Katharina Paschinger, Iain B H Wilson
Jan 1, 2016·PLoS Neglected Tropical Diseases·Ernesto RodríguezTeresa Freire
Feb 24, 2016·Glycoconjugate Journal·Katharina Paschinger, Iain B H Wilson
May 3, 2019·Parasitology·Katharina Paschinger, Iain B H Wilson
Apr 9, 2019·Open Biology·Feifei ZhuKeping Chen
Apr 6, 2016·The Journal of Biological Chemistry·Umul Kulthum AhmedJohn G Raynes
Nov 1, 2018·Molecular & Cellular Proteomics : MCP·Alba HykollariKatharina Paschinger

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Methods Mentioned

BETA
gel filtration
glycosylation

Software Mentioned

ProteinProspector MS - Fit
MS
Digest

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