Mass spectrometric analysis of the N-glycoproteome in statin-treated liver cells with two lectin-independent chemical enrichment methods

International Journal of Mass Spectrometry
Haopeng XiaoRonghu Wu

Abstract

Protein N-glycosylation is essential for mammalian cell survival and is well-known to be involved in many biological processes. Aberrant glycosylation is directly related to human disease including cancer and infectious diseases. Global analysis of protein N-glycosylation will allow a better understanding of protein functions and cellular activities. Mass spectrometry (MS)-based proteomics provides a unique opportunity to site-specifically characterize protein glycosylation on a large scale. Due to the complexity of biological samples, effective enrichment methods are critical prior to MS analysis. Here, we compared two lectin-independent methods to enrich glycopeptides for the global analysis of protein N-glycosylation by MS. The first boronic acid-based enrichment (BA) method benefits from the universal and reversible interactions between boronic acid and sugars; the other method utilizes metabolic labeling and click chemistry (MC) to incorporate a chemical handle into glycoproteins for future affinity enrichment. We comprehensively compared the performance of the two methods in the identification and quantification of glycoproteins in statin-treated liver cells. Based on the current results, the BA method is more universal i...Continue Reading

References

Sep 15, 2000·Journal of Neurochemistry·M C RyanE M Shooter
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Howard C HangCarolyn R Bertozzi
Jun 21, 2006·Proteomics·Willy MorelleJean-Claude Michalski
Sep 9, 2006·Cell·Kazuaki Ohtsubo, Jamey D Marth
Jun 26, 2007·Bioscience, Biotechnology, and Biochemistry·Masatoshi Goto
Oct 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jeremy M BaskinCarolyn R Bertozzi
Oct 24, 2007·Nature Methods·Lukas KällMichael J MacCoss
Apr 30, 2008·Journal of Proteome Research·Xiuxia DuMary S Lipton
Nov 14, 2008·Molecular & Cellular Proteomics : MCP·Jiyang ZhangFuchu He
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Feb 24, 2009·Nature Methods·Ying ZengJames C Paulson
Dec 22, 2009·Chemical Communications : Chem Comm·Marjoke F DebetsFloris L van Delft
Oct 5, 2010·Bioconjugate Chemistry·Vu HongM G Finn
Dec 25, 2010·Cell·Edward L HuttlinSteven P Gygi
Jan 25, 2011·Angewandte Chemie·Tongzhong JuRichard D Cummings
May 7, 2011·Chemical Communications : Chem Comm·Montserrat ShelbourneAfaf H El-Sagheer
Oct 8, 2011·Current Opinion in Structural Biology·Flavio Schwarz, Markus Aebi
Oct 11, 2011·Nature Methods·Catharina SteentoftHenrik Clausen
Jan 16, 2013·Nature Reviews. Urology·Sarah GilgunnRichard J O'Kennedy
Nov 1, 1994·Journal of the American Society for Mass Spectrometry·J K EngJ R Yates
Apr 3, 2014·Molecular & Cellular Proteomics : MCP·Weixuan ChenRonghu Wu
Sep 23, 2014·Chemistry & Biology·Craig S McKay, M G Finn
May 28, 2015·Proceedings of the National Academy of Sciences of the United States of America·Tomasz KurconLara K Mahal

❮ Previous
Next ❯

Citations

Sep 18, 2020·Molecular & Cellular Proteomics : MCP·Nicholas M RileySharon J Pitteri
Feb 16, 2021·Molecular & Cellular Proteomics : MCP·Nicholas M RileySharon J Pitteri
May 29, 2021·Mass Spectrometry Reviews·Fangxu SunRonghu Wu
May 22, 2021·Biochimica Et Biophysica Acta. Biomembranes·Eugenia M RapoportNicolai V Bovin
Jul 17, 2018·Accounts of Chemical Research·Haopeng XiaoRonghu Wu
Oct 19, 2019·Analytical Chemistry·Suttipong SuttapitugsakulRonghu Wu
Oct 7, 2021·Electrophoresis·Cristian D Gutierrez-ReyesYehia Mechref

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.