Mass spectrometric and computational analysis of cytokine-induced alterations in the astrocyte secretome.

Proteomics
Sarah Dunn KeeneHarry Ischiropoulos

Abstract

The roles of astrocytes in the CNS have been expanding beyond the long held view of providing passive, supportive functions. Recent evidence has identified roles in neuronal development, extracellular matrix maintenance, and response to inflammatory challenges. Therefore, insights into astrocyte secretion are critically important for understanding physiological responses and pathological mechanisms in CNS diseases. Primary astrocyte cultures were treated with inflammatory cytokines for either a short (1 day) or sustained (7 days) exposure. Increased interleukin-6 secretion, nitric oxide production, cyclooxygenase-2 activation, and nerve growth factor (NGF) secretion confirmed the astrocytic response to cytokine treatment. MS/MS analysis, computational prediction algorithms, and functional classification were used to compare the astrocyte protein secretome from control and cytokine-exposed cultures. In total, 169 secreted proteins were identified, including both classically and nonconventionally secreted proteins that comprised components of the extracellular matrix and enzymes involved in processing of glycoproteins and glycosaminoglycans. Twelve proteins were detected exclusively in the secretome from cytokine-treated astrocyt...Continue Reading

References

Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A GuidottiE Costa
Oct 12, 2001·Glia·Y Dong, E N Benveniste
Nov 10, 2001·Science·D H MauchF W Pfrieger
Aug 15, 2002·Genome Research·Juri RappsilberMatthias Mann
Nov 15, 2002·The Journal of Biological Chemistry·Wei-Qin ZhaoDaniel L Alkon
Dec 7, 2002·Nature Neuroscience·Micaela ZontaGiorgio Carmignoto
Dec 17, 2002·Nature Cell Biology·Nirit Mor-VakninDavid M Markovitz
Apr 24, 2003·The Journal of Biological Chemistry·Mireille Lafon-CazalPhilippe Marin
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
Jan 1, 2004·Progress in Brain Research·Pablo Villoslada, Claude P Genain
Apr 30, 2004·Protein Engineering, Design & Selection : PEDS·Jannick Dyrløv BendtsenSøren Brunak
Jun 25, 2004·Nucleic Acids Research·Giridhar ChukkapalliShankar Subramaniam
Sep 10, 2004·Nature·Sean J Mulligan, Brian A MacVicar
Oct 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yann BernardinelliJean-Yves Chatton
Oct 21, 2004·Nature Neuroscience·A Denise R GarciaMichael V Sofroniew
Dec 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jaime ImitolaSamia J Khoury
Feb 15, 2005·Cell·Karen S ChristophersonBen A Barres
Apr 6, 2005·Journal of Molecular Biology·Rajesh Nair, Burkhard Rost
Apr 23, 2005·Glia·Milos Pekny, Michael Nilsson
Jun 10, 2005·Journal of Neurology, Neurosurgery, and Psychiatry·M CombrinckL Minghetti
Jun 15, 2005·Journal of Proteome Research·Jae-Won YangGert Lubec
Jun 28, 2005·Molecular & Cellular Proteomics : MCP·William M OldNatalie G Ahn
Jul 19, 2005·Nature Reviews. Neuroscience·Andrea Volterra, Jacopo Meldolesi
Oct 8, 2005·Science·Olivier PascualPhilip G Haydon
Mar 11, 2006·Circulation Research·Jun SuzukiBradford C Berk
Mar 29, 2006·Journal of Bioinformatics and Computational Biology·John Hawkins, Mikael Bodén
Oct 13, 2006·Journal of Neurochemistry·Carole EscartinGilles Bonvento
Feb 15, 2007·Molecular Systems Biology·Michael W SchmidtDieter A Wolf
Mar 3, 2007·Drug Discovery Today·Eric W Klee, Carlos P Sosa
Apr 17, 2007·Nature Neuroscience·Francesco Paolo Di GiorgioKevin Eggan

❮ Previous
Next ❯

Citations

Aug 30, 2011·Analytical Chemistry·J TanneF Lisdat
Dec 4, 2010·Journal of Proteome Research·Nagarjuna Nagaraj, Matthias Mann
Jan 3, 2013·Science Signaling·Paschalis-Thomas DouliasHarry Ischiropoulos
May 5, 2010·The Journal of Clinical Investigation·Emma H WilsonChristopher A Hunter
Jul 4, 2012·Journal of Neuroinflammation·Hyejin JeonKyoungho Suk
Nov 13, 2012·Fluids and Barriers of the CNS·Anny-Claude LuissintPierre-Olivier Couraud
Dec 11, 2013·PloS One·Ling WangRamani Ramchandran
Jun 12, 2013·Journal of Proteomics·Paschalis-Thomas DouliasHarry Ischiropoulos
May 24, 2011·Journal of Proteomics·Ravindra V PolisettyRavi Sirdeshmukh
Dec 1, 2010·Translational Stroke Research·Jia LiuEdward P Feener
Mar 6, 2016·Journal of Neuroimmunology·Ivana RajkovicEmmanuel Pinteaux
Feb 19, 2013·Expert Review of Proteomics·Mithilesh Kumar Jha, Kyoungho Suk
Sep 10, 2014·Journal of Neuroinflammation·Jennifer L Furman, Christopher M Norris
Dec 28, 2012·Biochimica Et Biophysica Acta·Mithilesh Kumar JhaKyoungho Suk
Dec 4, 2015·International Journal of Molecular Sciences·Daisuke Ibi, Kiyofumi Yamada
Oct 24, 2009·Molecular and Cellular Neurosciences·Ethan G HughesRita J Balice-Gordon
Jun 16, 2009·Toxicology and Applied Pharmacology·Gennaro GiordanoLucio G Costa
Oct 11, 2015·Neurobiology of Aging·Sung Min SonInhee Mook-Jung
Oct 15, 2013·Proteomics·Mithilesh Kumar JhaKyoungho Suk
Jul 15, 2015·Science Signaling·Karthik RajuHarry Ischiropoulos
Nov 5, 2013·Neuroscience Letters·Amy J Gleichman, S Thomas Carmichael
May 25, 2011·Free Radical Biology & Medicine·Christie J BrunoHarry Ischiropoulos
Nov 3, 2012·Methods : a Companion to Methods in Enzymology·Paschalis-Thomas DouliasHarry Ischiropoulos
Jan 20, 2018·Physiological Reviews·Alexei Verkhratsky, Maiken Nedergaard
Oct 4, 2017·Cell Communication and Signaling : CCS·Ana Isabel OliveiraBruno M Costa
Aug 14, 2019·International Journal of Molecular Sciences·Parkyong SongJong Hyuk Yoon
Feb 13, 2010·Journal of Proteome Research·Yang ZhangYouhe Gao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.