Mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-O-diglucosides in cabernet sauvignon (Vitis vinifera L.) grape berries

Journal of Agricultural and Food Chemistry
Ran-Ran XingQiu-Hong Pan

Abstract

It has been widely accepted that anthocyanidin 3,5-O-diglucosides do not exist in Vitis vinifera L. Cabernet Sauvignon (CS) berries. However, our anthocyanin analyses using HPLC-ESI-MS/MS detected the existence of a low level of anthocyanidin 3,5-O-diglucosides in the Cabernet Sauvignon grape berries grown in China. The authenticity of these samples was confirmed with microsatellite markers. The existence of anthocyanidin 3,5-O-diglucoside was further verified by the enzymatic evidence for the first time. Four putative 5-O-glucosyltransferase (5GT) genes were isolated from the Cabernet Sauvignon berries. The enzymatic analysis showed that a recombinant protein (designated as Vv5GT3) glucosylated the 3-O- and 5-O-positions of anthocyanidins and flavonols. A phylogenetic analysis revealed that this bifunctional enzyme belongs to the 5GT subfamily of UDP-glycosyltransferases. This finding brought a new understanding of the anthocyanins' profile and their biosynthesis in V. vinifera and would be helpful for further investigations of the mechanism of accumulation of anthocyanidin diglucosides in Cabernet Sauvignon berries in China's wine-producing regions.

References

Jul 1, 1995·Critical Reviews in Food Science and Nutrition·G Mazza
Jun 26, 1999·Genome Génome / Conseil National De Recherches Canada·K M SefcH Steinkellner
Dec 20, 1999·Journal of Agricultural and Food Chemistry·M M GiustiR E Wrolstad
Feb 19, 2004·Journal of Agricultural and Food Chemistry·Stéphane VidalGeorge Skouroumounis
Jun 10, 2005·Nature·Jun OgataMasahiko Suzuki
Oct 11, 2005·Trends in Plant Science·Claire M M GachonPatrick Saindrenan
Sep 28, 2006·Journal of Agricultural and Food Chemistry·Fulvio MattiviRiccardo Velasco
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Aug 1, 1996·Genome Génome / Conseil National De Recherches Canada·J E BowersC P Meredith
Nov 19, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fu-Liang HanChang-Qing Duan
Apr 3, 2009·Journal of Agricultural and Food Chemistry·László JánváryWilfried Schwab
Jun 19, 2010·International Journal of Molecular Sciences·Quan ZhaoJun Wang
Sep 28, 2010·Journal of Agricultural and Food Chemistry·Noelia Castillo-MuñozIsidro Hermosín-Gutiérrez
Dec 15, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fei HeChang-Qing Duan
Jun 13, 2012·Analytica Chimica Acta·Alexander Acevedo De la CruzTristan Richard
Jun 1, 2013·Journal of Agricultural and Food Chemistry·Na-Na LiangChang-Qing Duan
Oct 3, 2013·International Journal of Molecular Sciences·Riccardo FlaminiLuigi Bavaresco
Sep 1, 1993·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M R Thomas, N S Scott
Sep 12, 2014·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yingzhen YangGan-Yuan Zhong
Dec 1, 2011·Food Chemistry·Zhenchang LiangLailiang Cheng

❮ Previous
Next ❯

Citations

Jun 21, 2016·Rapid Communications in Mass Spectrometry : RCM·Si-Yu LiChang-Qing Duan
Nov 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·José Pérez-NavarroCédric Saucier
Dec 3, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Iva ŠikutenDarko Preiner
Dec 5, 2020·Foods·Vakarė MerkytėEmanuele Boselli

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Zentralblatt Für Bakteriologie, Parasitenkunde, Infektionskrankheiten Und Hygiene. Zweite Naturwissenschaftliche Abt.: Allgemeine, Landwirtschaftliche Und Technische Mikrobiologie
R NagyE Witkovszky
Journal of Experimental Botany
Kentaro MoriKatsumi Hashizume
© 2021 Meta ULC. All rights reserved