Mass spectrometric detection of the amino acid sequence polymorphism of the hepatitis C virus antigen

Journal of Virological Methods
A L KayshevaAlexander Archakov

Abstract

A method for detection and identification of the hepatitis C virus antigen (HCVcoreAg) in human serum with consideration for possible amino acid substitutions is proposed. The method is based on a combination of biospecific capturing and concentrating of the target protein on the surface of the chip for atomic force microscope (AFM chip) with subsequent protein identification by tandem mass spectrometric (MS/MS) analysis. Biospecific AFM-capturing of viral particles containing HCVcoreAg from serum samples was performed by use of AFM chips with monoclonal antibodies (anti-HCVcore) covalently immobilized on the surface. Biospecific complexes were registered and counted by AFM. Further MS/MS analysis allowed to reliably identify the HCVcoreAg in the complexes formed on the AFM chip surface. Analysis of MS/MS spectra, with the account taken of the possible polymorphisms in the amino acid sequence of the HCVcoreAg, enabled us to increase the number of identified peptides.

References

Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J BukhR H Miller
Dec 13, 2006·Proteomics·Alexander Ivanovich ArchakovVictor Gavrilovich Zgoda
Feb 19, 2011·Biomedit︠s︡inskai︠a︡ khimii︠a︡·A L KaĭshevaA I Archakov
Jan 30, 2013·Biosensors & Bioelectronics·Yuri Dmitrievich IvanovAlexander Ivanovich Archakov

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Citations

Jun 10, 2019·Bulletin of Experimental Biology and Medicine·A L KayshevaYu D Ivanov
Apr 13, 2018·International Journal of Molecular Sciences·Tatyana O PleshakovaYuri D Ivanov

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