Mass-spectrometric profiling of porphyrins in complex biological samples with fundamental, toxicological, and pharmacological applications

Analytical Biochemistry
Sarah A SullivanJennifer L DuBois

Abstract

Rapid, high-throughput, and quantitative evaluations of biological metabolites in complex milieu are increasingly required for biochemical, toxicological, pharmacological, and environmental analyses. They are also essential for the development, testing, and improvement of new commercial chemical products. We demonstrate the application of ultra-high performance liquid chromatography-mass spectrometry (uHPLC-MS), employing an electrospray ionization source and a high accuracy quadrupole time-of-flight mass analyzer, for the identification and quantification of a series of porphyrin derivatives in liver: a matrix of particular relevance in toxicological or pharmacological testing. Exact mass is used to identify and quantify the metabolites. Chromatography enhances sensitivity and alleviates potential saturation issues by fanning out the contents of a complex sample before their injection into the spectrometer, but is not strictly necessary for the analysis. Extraction and sample treatment procedures are evaluated and matrix effects discussed. Using this method, the known mechanism of action of a well-characterized porphyrinogenic agent was verified in liver extracts from treated rats. The method was also validated for use with ba...Continue Reading

References

Jun 1, 1995·Human & Experimental Toxicology·G García-VargasF De Matteis
Aug 5, 1999·Archives of Environmental Health·A I SeldénS Thunell
Sep 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Dong-Sun LeeC S Raman
Oct 19, 2006·The Journal of Biological Chemistry·Hazel R CorradiK Ravi Acharya
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·John D PhillipsJames P Kushner
Jul 7, 2007·Critical Reviews in Toxicology·Yasuhiro Masubuchi, Toshiharu Horie
Jan 27, 2010·Chemical Research in Toxicology·Andrew G Smith, George H Elder
Mar 17, 2010·Lancet·Hervé PuyJean-Charles Deybach
Jan 27, 2012·Rapid Communications in Mass Spectrometry : RCM·Christopher M BentonDonald J L Jones

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Citations

Oct 27, 2015·Journal of Separation Science·Somayeh SedaghatAli Mostafavi

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