Mass spectrometric sequencing and acylation character analysis of C-terminal anchoring segment from Influenza A hemagglutinin

European Journal of Mass Spectrometry
Marina V SerebryakovaStanislav G Markushin

Abstract

Influenza A virus hemagglutinin (HA) is a major envelope glycoprotein mediating viral and cell membrane fusion. HA is anchored in the viral envelope by a light HA(2) chain containing one transmembrane domain and a cytoplasmic tail. Three cysteine residues in the C-terminal region, one in the transmembrane domain and two in the cytoplasmic tail, are highly conserved and potentially palmitoylated in all HA subtypes. The HA(2) C- terminal anchoring segments were extracted to organic phase from the bromelain-digested viruses (subviral particles) of three strains: A/X-31 (H3 subtype), A/Puerto Rico/8/34 (H1 subtype) and A/FPV/Weybridge/34 (H7 subtype). Their primary structures were assessed by matrix-assisted laser desorption/ionization time-of-flight time-of- flight mass spectrometry (MALDI-ToF-ToF MS). Trypsin-type protease-cleaved peptides prevailed over bromelain- cleaved ones in the peptide mixtures. All of them included transmembrane domains. Several distinctive features of the C-terminal HA(2) peptides acylation character were discovered by MALDI-ToF MS: 1) the peptides isolated from the viruses, which were digested by bromelain in the absence of beta-mercaptoethanol, were predominantly triply acylated; 2) the peptides were a...Continue Reading

References

Dec 6, 1989·Biochimica Et Biophysica Acta·M F Schmidt
Aug 2, 1972·Nature: New Biology·C M Brand, J J Skehel
Feb 1, 1981·The Journal of General Virology·T A Dopheide, C W Ward
Jul 14, 1995·The Journal of Biological Chemistry·M Joseph, R Nagaraj
Apr 8, 1998·Anästhesiologie, Intensivmedizin, Notfallmedizin, Schmerztherapie : AINS·K Rowan
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·J K Taubenberger
Aug 31, 2000·Annual Review of Biochemistry·J J Skehel, D C Wiley
Jul 10, 2002·The Journal of Biological Chemistry·Xiquan LiangMarilyn D Resh
Nov 5, 2002·Current Opinion in Chemical Biology·Wenjun Mo, Barry L Karger
Dec 14, 2002·Trends in Cell Biology·Marie-José Bijlmakers, Mark Marsh
Feb 8, 2003·The EMBO Journal·Christiane Kleuss, Eberhard Krause
Jan 20, 2004·Journal of Cell Science·Maurine E Linder, Robert J Deschenes
Aug 26, 2004·Protein and Peptide Letters·Larisa V KordyukovaLudmila A Baratova
Apr 21, 2005·Microbiology and Immunology·Makoto UjikeEri Nobusawa

❮ Previous
Next ❯

Citations

Nov 21, 2008·Journal of the American Society for Mass Spectrometry·Ning LiuZongwei Cai
Sep 12, 2008·Mass Spectrometry Reviews·Kevin M DownardAlexander B Schwahn
May 3, 2012·Biology of the Cell·Michael Veit
Jan 30, 2013·Biochemical Society Transactions·Michael VeitLarisa V Kordyukova
Sep 1, 2016·Toxicon : Official Journal of the International Society on Toxinology·Catherine A VulfiusYuri N Utkin
Jan 1, 2014·European Journal of Mass Spectrometry·Vladimir G Zaikin, Alexander A Sysoev
Nov 15, 2006·Journal of Mass Spectrometry : JMS
Aug 5, 2008·Bioorganicheskaia khimiia·Iu A SmirnovaL A Baratova
May 5, 2011·The Journal of Biological Chemistry·Marina V SerebryakovaVadim M Govorun
Jun 5, 2019·Protein and Peptide Letters·Larisa V KordyukovaMichael Veit
Feb 26, 2021·Cellular Microbiology·Mohamed Rasheed GadallaMichael Veit
Jul 22, 2021·Biochemistry. Biokhimii︠a︡·Larisa V Kordyukova, Andrey V Shanko
Dec 23, 2020·Journal of Proteome Research·Jinming ZhangNing Liu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.