Mass-spectrometrical analysis of proteins encoded on chromosome 21 in human fetal brain

Amino Acids
J-H ShinG Lubec

Abstract

Overexpression of chromosome 21 genes is directly or indirectly responsible for the Down syndrome phenotype. In order to analyse chromosome 21 gene products (Chr21Ps), we extracted proteins from fetal human brain cortex and applied an ultracentrifugal and chromatographic prefractionation principle followed by two-dimensional gel electrophoresis (2-DE) and mass-spectrometrical analysis using high-throughput automated MALDI-TOF/TOF. Nine Chr21Ps were identified: pyridoxal kinase; superoxide dismutase [Cu/Zn] 1; carbonyl reductase 1; ES1 protein homolog, mitochondrial [Precursor]; cystathionine-beta-synthetase; T-complex protein 1, theta subunit; cystatin B; 6-phosphofructokinase; glycinamide ribonucleotide synthetase. Mass-spectrometric characterisation of Chr21Ps following separation in 2-DE gels is a useful tool for the analysis of these structures in brain, independent of antibody availability and specificity.

References

Jul 22, 1991·FEBS Letters·V Turk, W Bode
Sep 30, 1985·Biochemical and Biophysical Research Communications·A RitonjaA J Barrett
Nov 5, 1999·Electrophoresis·K KarlssonM Fountoulakis
Jun 1, 2000·Nature·M HattoriUNKNOWN Chromosome 21 mapping and sequencing consortium
Mar 10, 2001·Journal of Neurochemistry·P J Kingham, J M Pocock
Jun 8, 2001·Annual Review of Biochemistry·M MannA Pandey
Feb 23, 2002·Amino Acids·E Engidawork, G Lubec
Oct 17, 2002·Journal of Neurology·Gert Lubec, Ephrem Engidawork
Nov 21, 2002·Proteomics·Michael FountoulakisGert Lubec
Dec 6, 2002·Nature·Alexandre ReymondAndrea Ballabio
Dec 6, 2002·Nature·Yorick GittonUNKNOWN HSA21 expression map initiative
Feb 27, 2003·Journal of Neurochemistry·Ephrem Engidawork, Gert Lubec
May 22, 2003·Progress in Neurobiology·Gert LubecMichael Fountoulakis
Aug 7, 2003·Biochemical and Biophysical Research Communications·Karin BrännvallDan Lindholm
Aug 19, 2003·Proteomics·Pier Giorgio RighettiJoël S Rossier
Mar 30, 2004·Proteomics·Rui VitorinoFrancisco M L Amado
Apr 8, 2004·Journal of Neural Transmission. Supplementum·G LubecL H Matherly
Apr 8, 2004·Journal of Neural Transmission. Supplementum·R Ferrando-MiguelG Lubec
Apr 20, 2004·Electrophoresis·Pierre LescuyerJean-Charles Sanchez

❮ Previous
Next ❯

Citations

Aug 24, 2010·Journal of Negative Results in Biomedicine·Frances K WisemanElizabeth M C Fisher
Jun 26, 2010·Neurochemical Research·Chenggang Zhang
Jul 18, 2009·Neuropharmacology·Lin LiGert Lubec
Nov 22, 2013·Proteomics. Clinical Applications·Marzia PerluigiD Allan Buttterfield
Jul 11, 2012·Molecular BioSystems·Chih-Ping ChenHong-Lin Chan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.