Mass Spectrometry-Based Analytical Strategy for Comprehensive Molecular Characterization of Biodegradable Poly(lactic-co -glycolic Acid) Copolymers

Journal of the American Society for Mass Spectrometry
Thierry N J FouquetLaurence Charles

Abstract

An analytical methodology with mass spectrometry as the core technique was developed for precise characterization of end groups, size, and co-monomeric composition of poly(lactic-co-glycolic acid) (PLGA) copolymers, as a preliminary step to qualify their biodegradability. Four PLGA samples were studied, with GA molar content varying from 0 to 50% and Mw ranging from 18 to 75 kg mol-1 according to the supplier. Size exclusion chromatography (SEC) and liquid state nuclear magnetic resonance (NMR) were used as either complementary or validation techniques. As confirmed by tandem mass spectrometry (MS/MS) experiments, macrocycles were most prominent in the low mass range. Nevertheless, elemental compositions derived from high resolution (HR) mass measurements of linear species were consistent with chain terminations revealed by NMR. Off-line coupling of SEC with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) permitted calibration curves to be built based on absolute molecular weights and, although slightly overestimated, so-obtained Mn and Mw values compared well with SEC and NMR results. Homogeneity of the co-monomeric content of all chains within each PLGA sample was demonstrated using surface-assisted l...Continue Reading

References

Oct 10, 2002·Mass Spectrometry Reviews·Maurizio S Montaudo
Oct 26, 2005·Journal of the American Society for Mass Spectrometry·Takaya SatohYoshihiro Kammei
Jul 14, 2010·Mass Spectrometry Reviews·Chrys WesdemiotisBryan C Katzenmeyer
Nov 14, 2012·Journal of Mass Spectrometry : JMS·Vincenzo Scionti, Chrys Wesdemiotis
Aug 5, 2018·Rapid Communications in Mass Spectrometry : RCM·Yasuhide NaitoTakayuki Ohmura
Jan 16, 2016·Polymers·Iman ManavitehraniFariba Dehghani
Nov 24, 2019·Journal of Mass Spectrometry : JMS·Thierry N J Fouquet
Nov 25, 2019·Rapid Communications in Mass Spectrometry : RCM·Steffen M Weidner, Hans R Kricheldorf
Dec 14, 2019·Rapid Communications in Mass Spectrometry : RCM·Paola RizzarelliGraziella Valenti

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