Mass Spectrometry-Based Biomarker Discovery

Methods in Molecular Biology
Weidong ZhouCaterina Longo

Abstract

The discovery of candidate biomarkers within the entire proteome is one of the most important and challenging goals in proteomic research. Mass spectrometry-based proteomics is a modern and promising technology for semiquantitative and qualitative assessment of proteins, enabling protein sequencing and identification with exquisite accuracy and sensitivity. For mass spectrometry analysis, protein extractions from tissues or body fluids and subsequent protein fractionation represent an important and unavoidable step in the workflow for biomarker discovery. Following extraction of proteins, the protein mixture must be digested, reduced, alkylated, and cleaned up prior to mass spectrometry. The aim of our chapter is to provide comprehensible and practical lab procedures for sample digestion, protein fractionation, and subsequent mass spectrometry analysis.

Citations

Mar 25, 2019·Future Science OA·Lorena HänelHartmut Schlüter
Aug 8, 2020·Annals of the New York Academy of Sciences·Naixuan DongZhengwei Yuan
Mar 14, 2019·International Journal of Legal Medicine·Kyoung-Min ChoiStefan Pittner
Oct 22, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sachio TsuchidaTomohiro Nakayama
Dec 19, 2020·Journal of Cellular and Molecular Medicine·Shuang-Shuang GuJun Wang
Mar 25, 2021·Mass Spectrometry Reviews·Erika PonziniRita Grandori
Jul 1, 2021·Journal of the American Society for Mass Spectrometry·Jingwei Cai, Zhengyin Yan

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