Mass spectrometry based proteomics for absolute quantification of proteins from tumor cells

Methods : a Companion to Methods in Enzymology
Hong Wang, S M Hanash

Abstract

In-depth quantitative profiling of the proteome and sub-proteomes of tumor cells has relevance to tumor classification, the development of novel therapeutics, and of prognostic and predictive markers and to disease monitoring. In particular the tumor cell surface represents a highly relevant compartment for the development of targeted therapeutics and immunotherapy. We have developed a proteomic platform to profile tumor cells that encompasses enrichment of surface membrane proteins, intact protein fractionation and label-free mass spectrometry based absolute quantification. Here we describe the methodology for capture, identification and quantification of cell surface proteins using biotinylation for labeling of the cell surface, avidin for capture of biotinylated proteins and ion mobility mass spectrometry for protein identification and quantification.

References

Apr 20, 2007·Journal of Proteome Research·Victor P AndreevBarry L Karger
May 20, 2011·Proteomics·André KriegeskorteKarsten Becker
Aug 14, 2013·PloS One·Enrica EleuterioStefania Angelucci

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Citations

Oct 21, 2015·Drug Discovery Today·Matheus H DiasLeo K Iwai
Jun 5, 2015·Methods : a Companion to Methods in Enzymology·Benoit Coulombe, Marie-Soleil Gauthier
May 28, 2019·International Journal of Molecular Sciences·Cristina Alexandra Ciocan-CartitaIoana Berindan-Neagoe
Nov 25, 2016·Proteomes·Trevor T Duarte, Charles T Spencer
Dec 30, 2017·Expert Review of Proteomics·Merry L LindseyKristine Y DeLeon-Pennell
Jun 12, 2021·Journal for Immunotherapy of Cancer·Hiroyuki KatayamaSam Hanash

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