Mass Spectrometry Imaging and Identification of Peptides Associated with Cephalic Ganglia Regeneration in Schmidtea mediterranea.

The Journal of Biological Chemistry
Ta-Hsuan OngJ V Sweedler

Abstract

Tissue regeneration is a complex process that involves a mosaic of molecules that vary spatially and temporally. Insights into the chemical signaling underlying this process can be achieved with a multiplex and untargeted chemical imaging method such as mass spectrometry imaging (MSI), which can enablede novostudies of nervous system regeneration. A combination of MSI and multivariate statistics was used to differentiate peptide dynamics in the freshwater planarian flatwormSchmidtea mediterraneaat different time points during cephalic ganglia regeneration. A protocol was developed to makeS. mediterraneatissues amenable for MSI. MS ion images of planarian tissue sections allow changes in peptides and unknown compounds to be followed as a function of cephalic ganglia regeneration. In conjunction with fluorescence imaging, our results suggest that even though the cephalic ganglia structure is visible after 6 days of regeneration, the original chemical composition of these regenerated structures is regained only after 12 days. Differences were observed in many peptides, such as those derived from secreted peptide 4 and EYE53-1. Peptidomic analysis further identified multiple peptides from various known prohormones, histone proteins...Continue Reading

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Citations

May 11, 2017·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Ying-Ning HoYu-Liang Yang
Mar 9, 2018·Journal of Proteome Research·Ning YangJonathan V Sweedler
Jul 28, 2020·Current Protein & Peptide Science·Madhuri JayathirthaCostel C Darie
Nov 18, 2020·Current Protein & Peptide Science·Nhu Q VuLingjun Li

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Methods Mentioned

BETA
Amputation
dissection
Fluorescence Imaging
PCA
acetylation
amidation
optical microscopy

Software Mentioned

PEAKS Studio
AxioVision
PEAKS
SignalP
flexImaging
ClinProTools
Mascot
BLAST
flexAnalysis

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