Mass Spectrometry Imaging (MSI) for Plant Metabolomics

Methods in Molecular Biology
Berin A Boughton, Dinaiz Thinagaran

Abstract

Mass spectrometry imaging (MSI) is a developing technique to measure the spatiotemporal distribution of many biomolecules in tissues. Over the preceding decade MSI has been adopted by plant biologists and applied in a broad range of areas including: primary metabolism, natural products, plant defense, plant responses to abiotic and biotic stress, plant lipids, and the developing field of spatial metabolomics. This methods chapter covers preparation of plant tissues for matrix-assisted laser desorption ionization (MALDI)-MSI, including sample embedding and freezing, sectioning, mounting, and matrix deposition using both sublimation and spray deposition prior to MSI analysis.

Citations

Aug 1, 2019·Natural Product Reports·Joseph E SprakerLaura M Sanchez
Oct 28, 2019·Journal of Mass Spectrometry : JMS·Berin A BoughtonAngus C Grey
Sep 10, 2019·Mass Spectrometry Reviews·Ana Margarida RodriguesCarla António
Mar 20, 2021·Frontiers in Plant Science·Zhiqiang PangYi Cheng
Jul 11, 2021·Journal of Separation Science·Wenya HuHonggang Nie

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