Mass spectrometry screening reveals widespread diversity in trichome specialized metabolites of tomato chromosomal substitution lines.

The Plant Journal : for Cell and Molecular Biology
Anthony L SchilmillerRobert L Last

Abstract

Glandular secreting trichomes of cultivated tomato (Solanum lycopersicum) and close relatives produce a variety of structurally diverse volatile and non-volatile specialized ('secondary') metabolites, including terpenes, flavonoids and acyl sugars. A genetic screen is described here to profile leaf trichome and surface metabolite extracts of nearly isogenic chromosomal substitution lines covering the tomato genome. These lines contain specific regions of the Solanum pennellii LA0716 genome in an otherwise 'wild-type' M82 tomato genetic background. Regions that have an impact on the total amount of extractable mono- and sesquiterpenes (IL2-2) or only sesquiterpenes (IL10-3) or specifically influence accumulation of the monoterpene alpha-thujene (IL1-3 and IL1-4) were identified using GC-MS. A rapid LC-TOF-MS method was developed and used to identify changes in non-volatile metabolites through non-targeted analysis. Metabolite profiles generated using this approach led to the discovery of introgression lines producing different acyl chain substitutions on acyl sugar metabolites (IL1-3/1-4 and IL8-1/8-1-1), as well as two regions that influence the quantity of acyl sugars (IL5-3 and IL11-3). Chromosomal region 1-1/1-1-3 was found ...Continue Reading

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Citations

Apr 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Alexander Weinhold, Ian Thomas Baldwin
Jul 14, 2012·Natural Product Research·Maria MinutoloAngela Errico
Dec 6, 2011·Journal of Experimental Botany·R Kooke, J J B Keurentjes
Jul 8, 2010·Plant Physiology·Geng YuEran Pichersky
Aug 5, 2011·Plant Physiology·Vasiliki FalaraEran Pichersky
Sep 19, 2012·Proceedings of the National Academy of Sciences of the United States of America·Anthony L SchilmillerRobert L Last
Jan 22, 2014·Journal of Experimental Botany·Takayuki TohgeAlisdair R Fernie
Dec 14, 2011·Plant & Cell Physiology·Keiichi Mochida, Kazuo Shinozaki
Feb 7, 2012·Plant Biotechnology Reports·Yozo Okazaki, Kazuki Saito
Dec 31, 2015·Proceedings of the National Academy of Sciences of the United States of America·Pengxiang FanRobert L Last
Jun 21, 2014·Trends in Plant Science·Biswapriya B MisraSixue Chen
Sep 18, 2012·Plant Biotechnology Journal·B Markus Lange, Glenn W Turner
Aug 12, 2014·Journal of Integrative Plant Biology·Kevin SchwahnTakayuki Tohge
Jan 9, 2013·Current Opinion in Plant Biology·Daniel J Kliebenstein
May 22, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiaojian ShaoHongxia Liu
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Jan 1, 2012·Metabolites·Eve Syrkin WurteleBasil J Nikolau
Sep 7, 2017·Annual Review of Genetics·Alisdair R Fernie, Takayuki Tohge
May 8, 2018·The Plant Journal : for Cell and Molecular Biology·Saleh Alseekh, Alisdair R Fernie
Aug 11, 2017·Molecular Nutrition & Food Research·Morgan J CichonSteven J Schwartz

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Methods Mentioned

BETA
NMR

Software Mentioned

MarkerLynx
QuanLynx
OPLS
DA

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