Mass spectroscopic characteristics of low molecular weight proteins extracted from calcium oxalate stones: preliminary study.

Journal of Clinical Laboratory Analysis
Wen-Chi ChenFuu-Jen Tsai

Abstract

It is believed that boundary compositions of matrix proteins might play a role in stone formation; however, few proteomic studies concerning matrix proteins in urinary stones have been conducted. In this study, we extracted low molecular weight proteins from calcium oxalate stones and measured their characteristic patterns by mass spectroscopy. A total of 10 stones were surgically removed from patients with urolithiasis. Proteins were extracted from the stones and identified by one-dimensional electrophoresis (sodium dodecyl sulfate buffer [SDS]-polyacrylamide gel electrophoresis [SDS-PAGE]). After in-gel digest, samples were analyzed by the surface-enhanced laser desorption ionization-time of flight (SELDI-TOF) technique. The peptide sequences were analyzed from the data of mass spectroscopy. Proteins were identified from Database Search (SwissProt Protein Database; Swiss Institute of Bioinformatics; http://www.expasy.org/sprot) on a MASCOT server (Matrix Science Ltd.; http://www.matrixscience.com). A total of three bands of proteins (27, 18, and 14 kDa) were identified from SDS-PAGE in each stone sample. A database search (SwissProt) on a MASCOT server revealed that the most frequently seen proteins from band 1 (27 kDa) were ...Continue Reading

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Citations

Sep 5, 2009·Urological Research·Benjamin K CanalesManoj Monga
Feb 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Karen S SfanosWilliam B Isaacs
Jul 23, 2013·PloS One·Nobuaki OkumuraToshifumi Takao
Apr 9, 2013·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Alan NgLyndon Jones
Apr 13, 2012·International Journal of Urology : Official Journal of the Japanese Urological Association·Kiyoko KanekoToru Shimizu
Oct 15, 2013·International Journal of Urology : Official Journal of the Japanese Urological Association·Kiyoko KanekoKoji Suzuki
Aug 17, 2010·Urology·Benjamin K CanalesManoj Monga
Dec 18, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Chanchai BoonlaKerstin Krieglstein
Mar 6, 2012·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Alan NgLyndon Jones
Sep 12, 2015·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kiyoko KanekoShigeo Horie
Jul 29, 2021·Expert Review of Proteomics·Paleerath Peerapen, Visith Thongboonkerd
Aug 15, 2014·Journal of the American Chemical Society·Sahar FarmaneshJeffrey D Rimer

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