PMID: 3768332Sep 9, 1986Paper

Massive phosphorylation distinguishes Xenopus laevis nucleoplasmin isolated from oocytes or unfertilized eggs

Biochemistry
M CottenR Chalkley

Abstract

Nucleoplasmin isolated from unfertilized Xenopus laevis eggs possesses an in vitro chromatin assembly activity which is superior to nucleoplasmin isolated from oocytes. It is demonstrated here that the two forms of the protein differ in the amount of attached phosphate, with the egg protein possessing nearly 20 phosphate groups per protein monomer and the oocyte protein possessing less than 10 phosphate groups per monomer. A kinase preparation from unfertilized eggs is shown to be capable of modifying oocyte nucleoplasmin so that it displays the electrophoretic heterogeneity of egg nucleoplasmin. Furthermore, when the egg protein is treated with phosphatase and repurified, the chromatin assembly activity deteriorates to the level of the oocyte protein.

References

May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J E GermondP Chambon
Jul 15, 1977·Developmental Biology·J MallerJ C Gerhart
Jan 25, 1985·Nucleic Acids Research·M Cotten, R Chalkley
May 20, 1986·Biochemistry·L SealyR Chalkley
Mar 1, 1969·Archives of Biochemistry and Biophysics·S Panyim, R Chalkley
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J M StadelR J Lefkowitz
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G Krohne, W W Franke

❮ Previous
Next ❯

Citations

May 1, 1995·Journal of Cellular Biochemistry·P L PaineN Sahay
Nov 19, 2003·Journal of Molecular Biology·Sonia BañuelosArturo Muga
Aug 11, 2010·The Journal of Biological Chemistry·Isbaal RamosAdelina Prado
Jul 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Adelina PradoJuan Ausió
Jan 25, 2014·Biochimica Et Biophysica Acta·Simon J Elsässer, Sheena D'Arcy
Aug 18, 2009·Journal of Molecular Biology·Stefka G TanevaMaría A Urbaneja
Oct 18, 2006·Molecular Reproduction and Development·Alejandra M VitaleScott Coonrod
Dec 15, 2010·Biochemistry Research International·Mikael S Lindström
Jun 7, 2002·Molecular Cell·Dominique Ray-GalletGeneviève Almouzni
Mar 29, 2000·Seminars in Cell & Developmental Biology·A PhilpottR A Laskey
Aug 26, 2016·Scientific Reports·Noelia Fernández-RiveroAdelina Prado
Apr 3, 1990·Biochemistry·J L Maller
May 4, 1993·Biochemistry·A R SburlatiS L Berger
Mar 29, 1993·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R A LaskeyC Dingwall
Dec 26, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Lindsay J FrehlickJuan Ausió
Aug 15, 1988·European Journal of Biochemistry·M S ErardF Amalric
May 19, 2007·The Journal of Biological Chemistry·Sonia BañuelosArturo Muga
Oct 4, 1996·The Journal of Biological Chemistry·T ItoJ T Kadonaga
Mar 29, 2002·The Journal of Biological Chemistry·Michael A Myre, Danton H O'Day
Mar 29, 1996·The Journal of Biological Chemistry·G H LenoR A Laskey
Nov 11, 2019·Cell Stress & Chaperones·Ashish Kumar, Dileep Vasudevan
Apr 15, 2000·Journal of Molecular Biology·P RodriguezM Zannis-Hadjopoulos
May 8, 2021·Journal of Cellular Physiology·Fateme Karimi DermaniRazieh Amini
Jul 10, 1992·Cell·U T Meier, G Blobel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.