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Massively differential bias between two widely used Illumina library preparation methods for small RNA sequencing

bioRxiv

Dec 19, 2013

Jeanette Baran-GalePraveen Sethupathy

Abstract

Recent advances in sequencing technology have helped unveil the unexpected complexity and diversity of small RNAs. A critical step in small RNA library preparation for sequencing is the ligation of adapter sequences to both the 5’ and 3’ ends of small RNAs. Two widely used protocols for...read more

Mentioned in this Paper

Study
Small Nuclear RNA
RNA library
Sequence Determinations, RNA
Research Personnel
Nucleic Acid Sequencing
Laboratory
Sequencing
tris(4,7-diphenyl-1,10-phenanthroline)ruthenium (II)
Molecular Genetic Technique
27
33
Paper Details
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Massively differential bias between two widely used Illumina library preparation methods for small RNA sequencing

bioRxiv

Dec 19, 2013

Jeanette Baran-GalePraveen Sethupathy

PMID: 990001479

DOI: 10.1101/001479

Abstract

Recent advances in sequencing technology have helped unveil the unexpected complexity and diversity of small RNAs. A critical step in small RNA library preparation for sequencing is the ligation of adapter sequences to both the 5’ and 3’ ends of small RNAs. Two widely used protocols for...read more

Mentioned in this Paper

Study
Small Nuclear RNA
RNA library
Sequence Determinations, RNA
Research Personnel
Nucleic Acid Sequencing
Laboratory
Sequencing
tris(4,7-diphenyl-1,10-phenanthroline)ruthenium (II)
Molecular Genetic Technique
27
33

Similar Papers Found In These Feeds

RNAi & Gene Silencing (Preprints)

RNA Interference (RNAi) pathway is involved in post-transcriptional gene silencing, transcriptional silencing and epigenetic silencing as well as its use as a tool for forward genetics and therapeutics. Discover the latest preprints on RNAi here.

BioRxiv Preprints

BioRxiv is the preprint server for biology, operated by Cold Spring Harbor Laboratory. Here are the latest biology preprint articles from bioRxiv.

Related Papers

Cold Spring Harbor Protocols

High-throughput illumina strand-specific RNA sequencing library preparation

Cold Spring Harbor ProtocolsAugust 3, 2011
Silin ZhongJames J Giovannoni
Paper Details
References
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  • Citations
  • finger pointing at paper

    References currently unavailable

    We're still populating references for this paper, please check back later.
  • References
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    No citations available

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