Massively parallel sequencing of 17 commonly used forensic autosomal STRs and amelogenin with small amplicons

Forensic Science International. Genetics
Eun Hye KimKyoung-Jin Shin

Abstract

The next-generation sequencing (NGS) method has been utilized to analyze short tandem repeat (STR) markers, which are routinely used for human identification purposes in the forensic field. Some researchers have demonstrated the successful application of the NGS system to STR typing, suggesting that NGS technology may be an alternative or additional method to overcome limitations of capillary electrophoresis (CE)-based STR profiling. However, there has been no available multiplex PCR system that is optimized for NGS analysis of forensic STR markers. Thus, we constructed a multiplex PCR system for the NGS analysis of 18 markers (13CODIS STRs, D2S1338, D19S433, Penta D, Penta E and amelogenin) by designing amplicons in the size range of 77-210 base pairs. Then, PCR products were generated from two single-sources, mixed samples and artificially degraded DNA samples using a multiplex PCR system, and were prepared for sequencing on the MiSeq system through construction of a subsequent barcoded library. By performing NGS and analyzing the data, we confirmed that the resultant STR genotypes were consistent with those of CE-based typing. Moreover, sequence variations were detected in targeted STR regions. Through the use of small-sized...Continue Reading

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Citations

Oct 23, 2016·Forensic Science International. Genetics·Xueying ZhaoWenbin Liu
Feb 23, 2020·Angewandte Chemie·Robert N GrassWendelin J Stark
Mar 28, 2017·Scientific Reports·Dame Loveliness T ApagaMaria Corazon A De Ungria
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Jan 4, 2019·International Journal of Legal Medicine·Jie WuYa-Jun Deng
Feb 26, 2021·Molecular Genetics & Genomic Medicine·Hui LiJianhui Xie

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