Massively Parallel Sequencing of Forensic STRs Using the Ion Chef™ and the Ion S5™ XL Systems

Journal of Forensic Sciences
Le WangJian Ye

Abstract

Next-generation sequencing (NGS) has been used to genotype forensic short tandem repeat (STR) markers for individual identification and kinship analysis. STR data from several NGS platforms have been published, but forensic application trials using the Ion S5™ XL system have not been reported. In this work, we report sensitivity, reproducibility, mixture, simulated degradation, and casework sample data on the Ion Chef™ and S5™ XL systems using an early access 25-plex panel. Sensitivity experiments showed that over 97% of the alleles were detectable with down to 62 pg input of genomic DNA. In mixture studies, alleles from minor contributors were correctly assigned at 1:9 and 9:1 ratios. NGS successfully gave 12 full genotype results from 13 challenging casework samples, compared with five full results using the CE platform. In conclusion, the Ion Chef™ and the Ion S5™ XL systems provided an alternative and promising approach for forensic STR genotyping.

References

Nov 17, 2007·Journal of Forensic Sciences·Carolyn R HillJohn M Butler
May 6, 2011·Forensic Science International. Genetics·Douglas R Hares
Apr 17, 2012·Forensic Science International. Genetics·Christophe Van NesteDieter Deforce
Jun 19, 2013·Forensic Science International. Genetics·David H WarshauerBruce Budowle
Dec 10, 2013·Forensic Science International. Genetics·Eszter RockenbauerNiels Morling
Dec 10, 2013·Forensic Science International. Genetics·S DalsgaardN Morling
Feb 18, 2014·Forensic Science International. Genetics·Christophe Van NesteFilip Van Nieuwerburgh
Dec 3, 2014·Forensic Science International. Genetics·Sarah L FordyceNiels Morling
Dec 3, 2014·Forensic Science International. Genetics·David H WarshauerBruce Budowle
Dec 3, 2014·Forensic Science International. Genetics·Christophe Van NesteFilip Van Nieuwerburgh
Dec 3, 2014·Genomics, Proteomics & Bioinformatics·Yaran YangJiangwei Yan
Dec 22, 2014·Forensic Science International. Genetics·Xiangpei ZengBruce Budowle
Dec 30, 2014·Forensic Science International. Genetics·Stefano VernarecciPaola Montagna
Feb 24, 2015·Forensic Science International. Genetics·Claus Børsting, Niels Morling
Jun 24, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·John M Butler
Jul 16, 2015·Nature·Hong MaShoukhrat Mitalipov
Aug 8, 2015·Forensic Science International. Genetics·Xueying ZhaoYuan Ping
Sep 24, 2015·Genomics, Proteomics & Bioinformatics·David H WarshauerBruce Budowle
Oct 5, 2015·Forensic Science International. Genetics·Jennifer D ChurchillBruce Budowle
Dec 25, 2015·Forensic Science International. Genetics·Katherine Butler GettingsPeter M Vallone
Jan 23, 2016·Forensic Science International. Genetics·Eun Hye KimKyoung-Jin Shin
Apr 16, 2016·Forensic Science International. Genetics·Fei GuoXianhua Jiang
Jun 28, 2016·Forensic Science International. Genetics·Yan MaZhi Yang
Jun 28, 2016·Forensic Science International. Genetics·Kristiaan J van der GaagPeter de Knijff
Oct 23, 2016·Forensic Science International. Genetics·Xueying ZhaoWenbin Liu
Oct 23, 2016·Forensic Science International. Genetics·So Yeun KwonKyoung-Jin Shin
Oct 23, 2016·Forensic Science International. Genetics·Nicole M M NovroskiBruce Budowle

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Citations

Apr 23, 2020·International Journal of Legal Medicine·Vincent ZvénigoroskyChristine Keyser

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