Massively parallel signature sequencing profiling of fetal human neural precursor cells

Stem Cells and Development
Jingli CaiMahendra S Rao

Abstract

We have examined gene expression in multipotent neural precursor cells (NPCs) derived from human fetal (f) brain tissue and compared its expression profiles with embryonic stem (ESC) cells, embryoid body cell (EBC), and astrocyte precursors using the technique of massively parallel signature sequencing (MPSS). Gene expression profiles show that fNPCs express core neural stem cells markers and share expression profiles with astrocyte precursor cells (APCs) rather than ESC or EBC. Gene expression analysis shows that fNPCs differ from other adult stem and progenitor cells in their marker expression and activation of specific functional networks such as the transforming growth factorbeta (TGFbeta) and Notch signaling pathways. In addition, our results allow us to identify novel genes expressed in fNPCs and provide a detailed profile of fNPCs.

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Citations

Jul 24, 2010·The Journal of Infectious Diseases·Sara Gianella, Douglas D Richman
Oct 25, 2008·BMC Developmental Biology·James T CampanelliYing Liu
Oct 31, 2007·PLoS Computational Biology·Gene W YeoFred H Gage
Aug 6, 2011·The Journal of Surgical Research·Marjun P DuldulaoJulio Garcia-Aguilar
May 5, 2009·Molecular and Cellular Neurosciences·Lori BennettLorraine Iacovitti
Feb 27, 2009·Brain Pathology·Yi Yuen WangMark Cook
Dec 17, 2014·PloS One·Cecilie Jonsgar SandbergIver A Langmoen

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