Mast cell beta-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities

The Journal of Immunology : Official Journal of the American Association of Immunologists
Linhua PangAlan J Knox

Abstract

Recent studies have shown that a lack of eosinophils in asthmatic airway smooth muscle (ASM) bundles in contrast to the large number of mast cells is a key feature of asthma. We hypothesized that this is caused by beta-tryptase, the predominant mast cell-specific protease, abrogating the eosinophil chemotactic activities of ASM cell-derived eosinophil chemoattractants such as eotaxin and RANTES. We studied the effect of beta-tryptase on the immunoreactivities of human ASM cell-derived and recombinant eotaxin and other recombinant chemokines that are known to be produced by human ASM cells. We report in this study that purified beta-tryptase markedly reduced the immunoreactivity of human ASM cell-derived and recombinant eotaxin, but had no effect on eotaxin mRNA expression. The effect was mimicked by recombinant human beta-tryptase in the presence of heparin and was reversed by heat inactivation and the protease inhibitor leupeptin, suggesting that the proteolytic activity of tryptase is required. beta-Tryptase also exerted similar effects on recombinant RANTES, but not on the other chemokines and cytokines that were screened. Furthermore, a chemotaxis assay revealed that recombinant eotaxin and RANTES induced eosinophil migrati...Continue Reading

References

Aug 1, 1992·The Journal of Experimental Medicine·Y KameyoshiJ M Schröder
Dec 1, 1992·Journal of Cellular Biochemistry·J LohiJ Keski-Oja
Jul 1, 1990·American Journal of Respiratory Cell and Molecular Biology·E K Tam, G H Caughey
Oct 1, 1991·The Journal of Allergy and Clinical Immunology·D H BroideS I Wasserman
Aug 1, 1991·The Journal of Clinical Investigation·S J RuossG H Caughey
Dec 1, 1996·American Journal of Respiratory and Critical Care Medicine·N BerkmanK F Chung
Jan 28, 1999·The Journal of Biological Chemistry·M PallaoroG H Caughey
Jun 3, 1999·American Journal of Respiratory and Critical Care Medicine·O GhaffarB Lamkhioued
Jul 22, 1999·The European Respiratory Journal·O KasselN Frossard
Nov 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·F J CulleyT J Williams
Jan 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L Pang, A J Knox
Nov 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A J KnoxL Pang
Mar 29, 2002·American Journal of Respiratory Cell and Molecular Biology·Alaina J AmmitReynold A Panettieri
May 22, 2002·European Journal of Pharmacology·Heloisa H A FerreiraGilberto De Nucci
May 28, 2002·The European Respiratory Journal·N G CarrollA L James
May 31, 2002·The New England Journal of Medicine·Christopher E BrightlingIan D Pavord
Jul 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aili L LazaarEllen Puré
Mar 22, 2003·American Journal of Respiratory and Critical Care Medicine·Johan C KipsRomain A Pauwels
Sep 23, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Patrick BergerJ Manuel Tunon de Lara
Oct 14, 2004·The Journal of Allergy and Clinical Immunology·Suzanne ZuyderduynKlaus F Rabe
May 10, 2005·American Journal of Respiratory and Critical Care Medicine·Christopher E BrightlingPeter Bradding

❮ Previous
Next ❯

Citations

Jun 19, 2012·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L J MarkwickS R Johnson
Sep 6, 2012·International Archives of Allergy and Immunology·Y C XiaG A Mackay
Mar 22, 2014·Journal of Allergy·H AlkhouriJ M Hughes
Jul 27, 2014·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Elaine Zayas Marcelino da SilvaConstance Oliver
Jan 6, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zhirong FuLars Hellman
Aug 3, 2019·The European Respiratory Journal·Gunnar Pejler
Aug 23, 2008·Archives of Dermatological Research·Ilkka T HarvimaAnita Naukkarinen
May 15, 2007·Immunological Reviews·George H Caughey
Nov 30, 2006·Allergology International : Official Journal of the Japanese Society of Allergology·Kenji Izuhara, Hirohisa Saito
Dec 26, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kichul ShinDavid M Lee
Dec 26, 2015·The Journal of Dermatology·Mireille-Maria Suttle, Ilkka T Harvima
Feb 13, 2018·Immunological Reviews·Peter Bradding, Gunnar Pejler
Sep 9, 2006·American Journal of Respiratory and Critical Care Medicine·Davinder KaurChristopher E Brightling
Dec 15, 2007·American Journal of Respiratory Cell and Molecular Biology·Charlotte S MullanLinhua Pang
Dec 26, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Neil HendersonSimon Richard Johnson
Apr 9, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·X TanJ M Hughes
Apr 23, 2013·Mediators of Inflammation·Petra Seidel, Michael Roth
Sep 9, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R SaundersC Brightling
Jun 18, 2017·Archivum Immunologiae Et Therapiae Experimentalis·Agata Matejuk
Mar 9, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C M H H T RobroeksE Dompeling
May 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Atsushi KatoKenji Matsumoto
Feb 13, 2018·Immunological Reviews·Kaori MukaiStephen J Galli
May 20, 2006·American Journal of Respiratory and Critical Care Medicine·Vivien ChanStuart J Hirst
Nov 3, 2010·Archives of Oral Biology·Pedro Paulo de Andrade SantosLélia Batista de Souza
Jun 12, 2010·Molecular Immunology·Kirstin M HeutinckAjda T Rowshani

❮ Previous
Next ❯

Related Concepts

Related Feeds

Asthma

This feed focuses in Asthma in which your airways narrow and swell. This can make breathing difficult and trigger coughing, wheezing and shortness of breath.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Related Papers

American Journal of Respiratory and Critical Care Medicine
O GhaffarB Lamkhioued
The Journal of Allergy and Clinical Immunology
C M LillyJ P Hanrahan
American Journal of Physiology. Lung Cellular and Molecular Physiology
Neil HendersonSimon Richard Johnson
Chemical Immunology and Allergy
Hirohisa Saito
© 2021 Meta ULC. All rights reserved