Mast cell-dependent allergic responses are inhibited by ethanolic extract of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) testa.

Journal of Agricultural and Food Chemistry
Hong-Jhang ChenWenchang Chiang

Abstract

Allergy is an immune dysfunction caused by degranulation from mast cells in the early phase and cytokine secretion in the late phase of the cell. The purpose of this study was to investigate the effects of adlay (Job's tears, Coix lachryma-jobi L. var. ma-yuen Stapf) testa against beta-hexosaminidase release as a marker of degranulation in rat basophilic leukemia (RBL)-2H3 cells. The ethyl acetate fraction from ethanolic extracts of adlay testa (ATE-EtOAc) exhibited potent inhibitory activity that suppressed degranulation from RBL-2H3 cells stimulated by 1 microM A23187. The 20%-80% EtOAc/Hex subfractions of ATE-EtOAc significantly inhibited histamine release with a IC(50) of 75-100 microg/mL. In addition, the ATE-EtOAc subfractions suppressed interleukin (IL)-4, IL-6, and tumor necrosis factor-alpha secretion in RBL-2H3 cells, indicating that adlay testa were able to inhibit cytokine secretion. In order to explore the inhibitory mechanism of adlay testa in mast cell degranulation, we examined the activation of intracellular signaling molecules. Adlay testa inhibited the phosphorylation ERK expression. Furthermore, the two major active compounds, 4-hydroxyacetophenone and p-coumaric acid, were isolated from the ATE-EtOAc subfra...Continue Reading

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Citations

May 13, 2011·Journal of Agricultural and Food Chemistry·Huang-Hui ChenChing-Chuan Kuo
Jul 13, 2012·Bioscience, Biotechnology, and Biochemistry·Jung-Min YooTack-Joong Kim
Sep 23, 2014·International Journal of Molecular Sciences·Huey-Chun HuangTsong-Min Chang
Mar 17, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Wan-Joong KimTack-Joong Kim
Apr 10, 2014·Journal of Traditional and Complementary Medicine·Wen-Hsin TsaiChiang-Ting Chien
Jun 18, 2015·Bioresource Technology·Mark Daniel G de LunaMing-Chun Lu
Jul 20, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hong-Jhang ChenWenchang Chiang
Jan 19, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bin LiYanjiang Qiao
Jul 30, 2019·Frontiers in Pharmacology·Weixiao AnShousong Cao
Feb 3, 2011·Journal of Agricultural and Food Chemistry·Cheng-Pei ChungYun-Lian Lin
Apr 10, 2014·Journal of Agricultural and Food Chemistry·Yung-Hung ChuJung-Feng Hsieh

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