Mast cell heterogeneity in human lung tissue.

Clinical Science
F J van OverveldG K Terpstra

Abstract

1. In this study mast cells were found to comprise 2.1% of total cells recovered by enzymatic digestion of human lung tissue. 2. This mast cell population consisted of 79% formalin-sensitive, Alcian Blue-positive mast cells and 21% formalin-insensitive, Alcian Blue-positive mast cells. 3. By the use of centrifugal elutriation and subsequent Percoll gradient centrifugation, separate mixed cell populations could be obtained in which the mast cell constituents were either of the formalin-sensitive or -insensitive type. 4. Cell suspensions in which formalin-sensitive cells comprised 97% of mast cells contained approximately 1.34 pg of histamine per mast cell, whereas in preparations in which mast cells were 84% formalin-resistant the histamine content was approximately 4.17 pg of histamine per mast cell. 5. The histamine release upon anti-immunoglobulin E challenge of formalin-sensitive mast cells was greater than the release by formalin-insensitive mast cells. 6. After challenge with opsonized zymosan, only formalin-sensitive mast cells were able to release histamine. 7. Leukotriene C4 release was observed when formalin-sensitive mast cells were challenged with anti-immunoglobulin E. Formalin-insensitive mast cells showed no relea...Continue Reading

Citations

Nov 15, 1994·Annals of the New York Academy of Sciences·M MurakamiK F Austen
Jun 13, 2001·Oncogene·F Mechta-GrigoriouM Yaniv
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeffrey R CrosbyNancy A Lee
Jun 9, 2001·The Journal of Allergy and Clinical Immunology·N A LeeJ J Lee
Nov 14, 1994·European Journal of Pharmacology·H YamamuraK Ohata

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