PMID: 8613667Feb 5, 1996Paper

Mast cell TNF mRNA expression in nasal mucosa demonstrated by in situ hybridization: a comparison of mast cell detection methods

Journal of Immunological Methods
W H AndersonD H Broide

Abstract

We have used 35S-labelled RNA probes to detect TNF cytokine gene expression in nasal mucosa derived from patients with perennial rhinitis. As mast cells comprise a minor component of the total cell population in nasal mucosa, additional methods are needed to determine whether mast cells contribute to the cytokine mRNA detected by in situ hybridization. We have combined in situ hybridization with alternate methods to detect mast cells (tryptase immunostaining or toluidine blue staining) and determined that in situ hybridization coupled with tryptase immunostaining provides optimal methods to detect mast cell cytokine gene expression in tissue sections. Using in situ hybridization and tryptase immunostaining, we demonstrate that mast cells in nasal mucosa can express TNF mRNA. However, the number of tryptase-, TNF+ cells (1.99 +/- 1.59 cells/mm2) exceeded the number of tryptase+, TNF+ mast cells (0.09 +/- cells/mm2). Mast cells thus comprised a subpopulation of the total number of TNF mRNA positive cells in nasal mucosa.

References

Oct 1, 1992·American Journal of Respiratory Cell and Molecular Biology·Y OhkawaraT Takishima
Nov 1, 1992·The Journal of Experimental Medicine·P BraddingC H Heusser
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·L J WalshG F Murphy
Oct 1, 1991·The Journal of Allergy and Clinical Immunology·D H BroideS I Wasserman
Nov 1, 1991·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S YingQ Hamid
Jan 1, 1988·The Journal of Experimental Medicine·A F LopezM A Vadas
Oct 1, 1993·American Journal of Respiratory Cell and Molecular Biology·S YingQ A Hamid

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Citations

Mar 14, 2001·American Journal of Respiratory Cell and Molecular Biology·D H BroideP Sriramarao
Oct 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Reid K IkedaDavid H Broide

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