Mast cells present protrusions into blood vessels upon tracheal allergen challenge in mice

PloS One
Oishee BoseMatthew F Krummel

Abstract

Mast cells (MC) and myeloid dendritic cells (DC) act proximally in detecting and processing antigens and immune insults. We sought to understand their comparative dynamic behavior with respect to the airway epithelium in the steady state and in response to an allergic stimulus in mouse trachea. We devised methods to label MC in living trachea and to demonstrate that MC and DC occupy distinct layers of the tracheal mucosa, with DC being closer to the lumen. DC numbers doubled after allergen challenge, but MC numbers remained stable. MC and DC migrated minimally in either steady state or allergen-challenge conditions, and their interactions with one another appeared to be stochastic and relatively infrequent. While DC, unlike MC, exhibited probing behaviors involving dendrites, these projections did not cross the epithelium into the airway lumen. MC typically were located too far from the epithelial surface to contact the tracheal lumen. However, MC had protrusions toward and into blood vessels, likely to load with IgE. Thus, DC and MC occupy distinct niches and engage in sessile surveillance in the mouse trachea. Little or no access of these cell types to the airway lumen suggests that trans-epithelial transport of proteins in t...Continue Reading

References

Sep 14, 1990·Journal of Immunological Methods·Y Aida, M J Pabst
May 1, 1996·American Journal of Respiratory Cell and Molecular Biology·D I BlythD Fattah
Feb 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kaoru HamadaLester Kobzik
Nov 16, 2004·Nature Immunology·Randall L LindquistMichel C Nussenzweig
Jun 22, 2005·Seminars in Immunology·Hendrik Jan de HeerBart N Lambrecht
Jun 27, 2006·Nature Reviews. Immunology·Ronald N GermainMichel C Nussenzweig
Dec 6, 2006·The Journal of Experimental Medicine·Marcello ChieppaRonald N Germain
May 10, 2011·Nature Immunology·Brandon M SullivanRichard M Locksley
Apr 4, 2012·Current Protocols in Cytometry·Emily E ThorntonMark R Looney
May 16, 2012·The Journal of Experimental Medicine·Emily E ThorntonMatthew F Krummel
Jun 30, 2014·Trends in Immunology·Efrat LelkesMatthew F Krummel

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Citations

Apr 8, 2017·Scientific Reports·Tibor Z VeresMarko Salmi
Dec 3, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tibor Z VeresRonald N Germain
Feb 13, 2018·Immunological Reviews·Shweta TikooWolfgang Weninger

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Methods Mentioned

BETA
confocal microscopy
transgenic
dissection

Software Mentioned

Imaris ( Bitplane
Zeiss Aim
Matlab Math Works )
Fiji Open Source

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