Mast cells stimulated by membrane-bound, but not soluble, steel factor are dependent on phospholipase C activation

Cellular and Molecular Life Sciences : CMLS
N Z TrieselmannS A Berger

Abstract

The steel factor (SLF) and c-Kit growth factor/receptor pair are key molecules governing mast cell development and survival. SLF is expressed on stromal cells as a membrane-bound molecule (mSLF) which can be cleaved by proteases to release a soluble form (sSLF). We investigated the importance of phospholipase C (PLC) activation in mast cells stimulated by sSLF and mSLF. PLC antagonists U73122, neomycin sulfate and oleic acid inhibited mast cell thymidine incorporation stimulated by mSLF, but not by sSLF. These antagonists suppressed sSLF-induced Ca2+ transients but did not significantly interfere with c-Kit phosphorylation or PLC-gamma2 recruitment. p85, the regulatory subunit of phosphatidylinositol 3-kinase (PI3-kinase), was found to be efficiently recruited to c-Kit following stimulation by sSLF or mSLF. However PKB/Akt, a kinase activated by PI3-kinase products, was phosphorylated following sSLF stimulation, but not with mSLF. Taken together, these studies demonstrate the importance of PLC activation by mSLF in supporting mast cells.

Citations

Dec 31, 2004·Stem Cells·Johan LennartssonR Shivakrupa
Apr 22, 2014·Immunology and Allergy Clinics of North America·Siham BibiMichel Arock
Jun 9, 2007·British Journal of Haematology·Alberto OrfaoUNKNOWN REMA
Oct 10, 2006·European Journal of Oral Sciences·Eleni GagariPetros D Damoulis
Sep 1, 2005·Biochemical and Biophysical Research Communications·Robert Roskoski
Jan 13, 2015·Cellular and Molecular Life Sciences : CMLS·Patrizia NigroGiulio Pompilio
May 9, 2019·Physiological Reviews·Julhash U Kazi, Lars Rönnstrand
Oct 18, 2012·Physiological Reviews·Johan Lennartsson, Lars Rönnstrand
Dec 15, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·Anne SimonowskiMichael Huber

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