PMID: 2099342Jan 1, 1990Paper

Mast cells, tissue histamine and eosinophils in early- and late-phase skin reactions: effects of a single dose of prednisolone

International Archives of Allergy and Applied Immunology
A HammarlundL Enerbäck

Abstract

Skin prick tests with allergen and histamine were performed on the volar aspect of the forearms in a double-blind, cross-over study with 40 mg of prednisolone and placebo in 16 pollen-allergic subjects. Skin biopsies were taken before any treatment and 15 min (group 1; n = 8) and 6 h (group 2; n = 8) after local challenge with allergen, corresponding to the timing of an early- and late-phase reaction. The specimens were used for the histological evaluation of mast cell and eosinophil density as well as for the determination of the histamine and protein content. The size of the induced weal and flare area as well as of any late-phase reaction was determined using digitized planimetry. The single dose of prednisolone, given 2 h prior to challenge, did not affect the size of the weal and flare response. Only 4 of the individuals developed a visible late-phase response. Eosinophils were virtually absent before allergen exposure, but were already present 15 min after allergen challenge, largely associated with the blood vessels, and were numerous at 6 h. There was, however, no relationship between eosinophil density and the presence or extent of any visual late phase. The mast cells/basophils showed a tendency to increase at the 6-h...Continue Reading

Citations

Jan 1, 1995·Archives of Dermatological Research·N HaasB M Czarnetzki
Feb 2, 2013·Clinical and Translational Allergy·Lucie HeinzerlingRichard Lockey
Apr 30, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A TomkinsonJ Tepper
Jan 19, 2012·The Laryngoscope·Federica Di Berardino, Antonio Cesarani

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