Mastitis pathogens present in bulk tank milk from seven dairy herds in the Waikato region, New Zealand

New Zealand Veterinary Journal
P Howard

Abstract

To identify and enumerate colony forming units (cfu) of mastitis pathogens in bulk tank milk (BTM) from pasture-fed New Zealand dairy cows in the Waikato region. BTM samples from seven seasonal-calving dairy herds in the Waikato region were collected monthly from August to December 2004 (cows calved during July-September). Milk samples were cultured on blood aesculin and MacConkey agar plates for 24 h, and the number of mastitis pathogens identified and counted. Colonies identified in BTM included aesculin-positive streptococci, Staphylococcus aureus, coagulase-negative staphylococci (CNS), and coliforms; counts ranged from zero to >1,000 cfu/ml. Counts>1,000 cfu/ml for total aesculin-positive streptococci, CNS and coliforms were present in 48%, 51% and 11% of BTM samples, respectively. Counts of Staph. aureus ranged from zero to 1,000 cfu/ml, but first appeared in BTM samples only in October. Staphylococcus aureus was repeatedly isolated in BTM from 4/7 farms during the testing period. Counts of mastitis pathogens in this study appeared high relative to interpretive criteria set by other workers, which may indicate a high prevalence of mastitis risk factors on these farms. Interpretation of results is difficult as aesculin-pos...Continue Reading

References

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Citations

Aug 7, 2007·New Zealand Veterinary Journal·A M J McFaddenM R Alley
Mar 3, 2009·New Zealand Veterinary Journal·A M J McFaddenA F Julian
Jun 13, 2009·New Zealand Veterinary Journal·M DenisB M Buddle
Aug 11, 2010·Preventive Veterinary Medicine·Richard G M Olde RiekerinkDave F Kelton
Jul 8, 2017·Critical Reviews in Food Science and Nutrition·Qianting OuZhenjiang Yao
Jan 6, 2009·The Journal of Dairy Research·Dusan RysanekVladimir Babak

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Methods Mentioned

BETA
flow cytometry

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