PMID: 9540824Apr 16, 1998Paper

Mastoparan induces an increase in cytosolic calcium ion concentration and subsequent activation of protein kinases in tobacco suspension culture cells

Biochimica Et Biophysica Acta
K TakahashiShoshi Muto

Abstract

Mastoparan induced a transient elevation of cytosolic free Ca2+ concentration ([Ca2+]cyt) in tobacco suspension culture cells. The mastoparan-induced [Ca2+]cyt elevation was inhibited by 8-(N,N-diethylamino)-octyl 3,4,5-trimethoxybenzoate-HCl and neomycin but not by depletion of extracellular Ca2+, suggesting that the elevation was the result of Ca2+ release from the intracellular stores caused by stimulation of phosphoinositide turnover. Hydrogen peroxide which has been shown to induce an oxidative burst in soybean cells by mastoparan treatment [L. Legendre, P.F. Heinstein, P.S. Low, Evidence for participation of GTP-binding proteins in elicitation of the rapid oxidative burst in cultured soybean cells, J. Biol. Chem., 267 (1992) 20140-20147], also induced a transient [Ca2+]cyt elevation in the tobacco cells. However, mastoparan did not induce an oxidative burst in the tobacco cells. Activation of a 50, a 75 and a 80 kDa protein kinases after the mastoparan-induced [Ca2+]cyt elevation was shown by an in-gel protein kinase assay. This activation was inhibited by neomycin, suggesting that the [Ca2+]cyt elevation is necessary for the mastoparan-induced activation of the protein kinases. The activation was inhibited also by pretre...Continue Reading

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Citations

Feb 8, 2007·Journal of Plant Research·Takahiro Hamada
Feb 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·A S.N. Reddy
Jul 3, 2004·Nature Reviews. Molecular Cell Biology·Giles E D Oldroyd, J Allan Downie
May 8, 2003·Biological Procedures Online·Axel Mithöfer, Christian Mazars
Jul 27, 2006·The New Phytologist·David LecourieuxAlain Pugin
Jan 9, 2009·The New Phytologist·Christian MazarsRaoul Ranjeva
Oct 7, 2004·Biochemical and Biophysical Research Communications·Tomonori KawanoShoshi Muto
Aug 2, 2003·Biochemical and Biophysical Research Communications·Tomonori KawanoFrédéric Lapeyrie
Dec 20, 2012·Annals of Botany·Zhenya P YordanovaElena T Iakimova
Jun 24, 2000·Nature·N PaulyC Mazars
Mar 12, 2013·Scientific Reports·Tomoki MatsudaTakeharu Nagai
Jan 9, 2014·In Vitro Cellular & Developmental Biology. Animal·Md Abdul HannanIl Soo Moon
Sep 4, 2014·Cellular and Molecular Neurobiology·Camilla Stampe Jensen, Hiroaki Misonou
Sep 30, 2009·Cellular and Molecular Neurobiology·Claudia Margarethe BittinsHans-Hermann Gerdes
Mar 9, 2020·Medical Molecular Morphology·Hidenori ItoKoh-Ichi Nagata

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