Matcha green tea (MGT) inhibits the propagation of cancer stem cells (CSCs), by targeting mitochondrial metabolism, glycolysis and multiple cell signalling pathways

Aging
Gloria BonuccelliMichael P Lisanti

Abstract

Matcha green tea (MGT) is a natural product that is currently used as a dietary supplement and may have significant anti-cancer properties. However, the molecular mechanism(s) underpinning its potential health benefits remain largely unknown. Here, we used MCF7 cells (an ER(+) human breast cancer cell line) as a model system, to systematically dissect the effects of MGT at the cellular level, via i) metabolic phenotyping and ii) unbiased proteomics analysis. Our results indicate that MGT is indeed sufficient to inhibit the propagation of breast cancer stem cells (CSCs), with an IC-50 of ~0.2 mg/ml, in tissue culture. Interestingly, metabolic phenotyping revealed that treatment with MGT is sufficient to suppress both oxidative mitochondrial metabolism (OXPHOS) and glycolytic flux, shifting cancer cells towards a more quiescent metabolic state. Unbiased label-free proteomics analysis identified the specific mitochondrial proteins and glycolytic enzymes that were down-regulated by MGT treatment. Moreover, to discover the underlying signalling pathways involved in this metabolic shift, we subjected our proteomics data sets to bio-informatics interrogation via Ingenuity Pathway Analysis (IPA) software. Our results indicate that MGT ...Continue Reading

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Citations

Mar 15, 2019·Critical Reviews in Food Science and Nutrition·Xiao-Yu XuHua-Bin Li
Mar 8, 2020·International Journal of Molecular Sciences·Claudia MusialMagdalena Gorska-Ponikowska
Jan 12, 2019·Critical Reviews in Food Science and Nutrition·Hao-Feng GuMin Du
Feb 8, 2020·Molecular Cancer·Mouradi El HoutAhmed Hamaï
Feb 13, 2020·Signal Transduction and Targeted Therapy·Liqun YangHongjuan Cui
Apr 17, 2020·Signal Transduction and Targeted Therapy·Liqun YangHongjuan Cui
Apr 4, 2021·Apoptosis : an International Journal on Programmed Cell Death·Michela RaimondiPatrizia Limonta
Apr 9, 2021·Life Sciences·Pawan Kumar Raghav, Zoya Mann
Oct 6, 2021·Food & Function·Lei ZhaoZhao-Jia Ge

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Methods Mentioned

BETA
ubiquitination

Clinical Trials Mentioned

NCT02321969

Software Mentioned

Ingenuity Pathway Analysis ( IPA )
96
XFe
Excel
Seahorse

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