Material-Dependent Formation and Degradation of Bone Matrix-Comparison of Two Cryogels

Bioengineering
Weidong WengSabrina Ehnert

Abstract

Cryogels represent ideal carriers for bone tissue engineering. We recently described the osteogenic potential of cryogels with different protein additives, e.g., platelet-rich plasma (PRP). However, these scaffolds raised concerns as different toxic substances are required for their preparation. Therefore, we developed another gelatin (GEL)-based cryogel. This study aimed to compare the two scaffolds regarding their physical characteristics and their influence on osteogenic and osteoclastic cells. Compared to the PRP scaffolds, GEL scaffolds had both larger pores and thicker walls, resulting in a lower connective density. PRP scaffolds, with crystalized calcium phosphates on the surface, were significantly stiffer but less mineralized than GEL scaffolds with hydroxyapatite incorporated within the matrix. The GEL scaffolds favored adherence and proliferation of the osteogenic SCP-1 and SaOS-2 cells. Macrophage colony-stimulating factor (M-CSF) and osteoprotegerin (OPG) levels seemed to be induced by GEL scaffolds. Levels of other osteoblast and osteoclast markers were comparable between the two scaffolds. After 14 days, mineral content and stiffness of the cryogels were increased by SCP-1 and SaOS-2 cells, especially of PRP scaf...Continue Reading

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Citations

Apr 4, 2021·International Journal of Molecular Sciences·Victor HäusslingSabrina Ehnert
Sep 16, 2021·Journal of Materials Science. Materials in Medicine·Weidong WengRomina H Aspera-Werz

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Methods Mentioned

BETA
FCS
PMA

Software Mentioned

ImageJ
BoneJ
GraphPad Prism
SAFIRE

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