Maternal age-dependent APC/C-mediated decrease in securin causes premature sister chromatid separation in meiosis II

Nature Communications
Ibtissem NabtiJohn Carroll

Abstract

Sister chromatid attachment during meiosis II (MII) is maintained by securin-mediated inhibition of separase. In maternal ageing, oocytes show increased inter-sister kinetochore distance and premature sister chromatid separation (PSCS), suggesting aberrant separase activity. Here, we find that MII oocytes from aged mice have less securin than oocytes from young mice and that this reduction is mediated by increased destruction by the anaphase promoting complex/cyclosome (APC/C) during meiosis I (MI) exit. Inhibition of the spindle assembly checkpoint (SAC) kinase, Mps1, during MI exit in young oocytes replicates this phenotype. Further, over-expression of securin or Mps1 protects against the age-related increase in inter-sister kinetochore distance and PSCS. These findings show that maternal ageing compromises the oocyte SAC-APC/C axis leading to a decrease in securin that ultimately causes sister chromatid cohesion loss. Manipulating this axis and/or increasing securin may provide novel therapeutic approaches to alleviating the risk of oocyte aneuploidy in maternal ageing.

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Citations

Jul 25, 2017·International Journal of Molecular Sciences·Jin-Mei Cheng, Yi-Xun Liu
Dec 16, 2017·Human Reproduction Update·Jessica GreaneyHayden Homer
May 13, 2020·Journal of Pregnancy·Mehdi MoradinazarEbrahim Shakiba
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Aug 17, 2021·Frontiers in Cell and Developmental Biology·Tereza PauerovaMartin Anger

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