Maternal control of suspensor programmed cell death via gibberellin signaling

Nature Communications
Ce ShiMeng-Xiang Sun

Abstract

Plant embryos are generated and develop in a stable and well-protected microenvironment surrounded by maternal tissue, which is vital for embryogenesis. However, the signaling mechanisms responsible for maternal tissue-to-proembryo communication are not well understood. Here, we report a pathway for maternal tissue-to-proembryo communication. We identify a DELLA protein, NtCRF1 (NtCYS regulative factor 1), which regulates suspensor programmed cell death (PCD). NtCRF1 can bind to the promoter of NtCYS and regulate the suspensor PCD-switch module NtCYS-NtCP14 in response to gibberellin (GA). We confirm that GA4, as a primary signal triggering suspensor PCD, is generated in the micropylar endothelium by the transient activation of NtGA3oxs in the maternal tissue. Thus, we propose that GA is a maternal-to-proembryo communication signal that is decoded in the proembryo by a GID1-CRF1-CYS-CP14 signaling cascade. Using this mode of communication, maternal tissue precisely controls the embryonic suspensor PCD and is able to nurse the proembryo in a stage-dependent manner.

References

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Citations

Dec 7, 2019·Physiologia Plantarum·Downs Jacob, Jones Brian
Oct 28, 2020·Nature Plants·Michael D Nodine
Aug 3, 2021·Journal of Experimental Botany·Xuemei ZhouMeng-Xiang Sun

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Datasets Mentioned

BETA
AB016084.1
PRJNA319578
GSE133373

Methods Mentioned

BETA
one-hybrid
immunoprecipitation
electrophoretic mobility shift assay
transgenic
RNA-seq
two-hybrid
Y2H
ChIP
PCRs
electrophoresis

Software Mentioned

GraphPad Prism
PlantPan
Cutadapt
RSEM

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