Maternal diabetes impairs gastrulation and insulin and IGF-I receptor expression in rabbit blastocysts

Endocrinology
Nicole RaminAnne Navarrete Santos

Abstract

Women with type 1 diabetes are subfertile. Diabetes negatively affects pregnancy by causing early miscarriage and poor prenatal outcomes. In this study we examine consequences of maternal type 1 diabetes on early embryo development, metabolic gene expression, and the pattern of insulin receptor (IR) and IGF-I receptor (IGF-IR) distribution in rabbit blastocysts. In female rabbits, type 1 diabetes was induced by alloxan treatment. Six-day-old blastocysts were recovered and assessed for receptor distribution and metabolic gene expression. In vitro culture of blastocysts was performed in medium containing 1 mm, 10 mm, or 25 mm glucose, simulating normo- and hyperglycemic developmental condition in vitro. The fertility rate of the diabetic rabbits clearly mirrored subfertility with a drop in blastocyst numbers by 40% (13.3 blastocysts in diabetic vs. 21.9 in control females). In blastocysts onset and progression of gastrulation was delayed and expression of IR and IGF-IR and their metabolic target genes (hexokinase, phosphoenolpyruvate carboxykinase), both in vivo and in vitro, was down-regulated. The amount of apoptotic cells in the embryonic disc was increased, correlating closely with the reduced transcription of the bcl-x(L) ge...Continue Reading

References

Jan 1, 1992·Annual Review of Physiology·S J Pilkis, D K Granner
Nov 1, 1991·Journal of Reproduction and Fertility·K H MoleyM P Diamond
May 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·V P Knutson
Jan 1, 1990·Reproduction, Fertility, and Development·L F Beebe, P L Kaye
May 1, 1989·Journal of Reproduction and Fertility·M P DiamondA H DeCherney
Feb 1, 1986·Biology of Reproduction·J V O'Fallon, R W Wright
Sep 1, 1984·Journal of Reproduction and Fertility·H J Leese, A M Barton
Aug 1, 1995·Anatomy and Embryology·C ViebahnM Hrabé de Angelis
Aug 1, 1996·Cytokine & Growth Factor Reviews·V A BlakesleyD Le Roith
Jun 1, 1997·Molecular Reproduction and Development·A D LightenG E Moore
Jan 1, 1997·Annual Review of Biochemistry·R W Hanson, L Reshef
May 1, 1997·Reviews of Reproduction·P L Kaye
Oct 30, 1998·Endocrine Reviews·W C DuckworthF G Hamel
Aug 10, 1999·Fertility and Sterility·B D Bavister
Aug 19, 2000·Cellular and Molecular Life Sciences : CMLS·E PeschkeD Peschke
Jan 28, 2003·Molecular Reproduction and Development·Silke Kietz, Bernd Fischer
May 20, 2003·Nature·Pere PuigserverBruce M Spiegelman
Nov 5, 2003·Developmental Biology·Franchesca D HoughtonHenry J Leese
Oct 29, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Anne Navarrete SantosBernd Fischer
Oct 29, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Anne Navarrete SantosBernd Fischer
Dec 27, 2005·Current Drug Targets. Immune, Endocrine and Metabolic Disorders·P G Quinn, D Yeagley
Jun 15, 2007·Current Opinion in Clinical Nutrition and Metabolic Care·Emmanuelle MeugnierHubert Vidal

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Citations

Nov 6, 2013·Nature Reviews. Endocrinology·Aritro SenNorbert Gleicher
May 15, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Bernd FischerVeronique Duranthon
Feb 22, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Pouneh MaraghechiElen Gócza
May 14, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Elisa HauckeAnne Navarrete Santos
Aug 11, 2011·Molecular and Cellular Endocrinology·René ThiemeAnne Navarrete Santos
Feb 3, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Jacqueline GürkeAnne Navarrete Santos
Nov 3, 2011·Reproduction in Domestic Animals = Zuchthygiene·L LlobatJ S Vicente
Jun 20, 2012·Frontiers in Neuroendocrinology·John O'Kusky, Ping Ye
Mar 13, 2012·Biochemical and Biophysical Research Communications·Julia M KnelangenAnne Navarrete Santos
Dec 3, 2016·Scientific Reports·Andrew M KelleherThomas E Spencer
May 5, 2017·Human Reproduction·Maria SchindlerAnne Navarrete Santos
Feb 3, 2018·Molecular Reproduction and Development·Alejandra Sánchez-SantosLuis A Baiza-Gutman
Sep 19, 2012·Obesity Facts·Cornelia Alice Irene KönigsdorfBernd Fischer
Feb 6, 2020·International Journal of Molecular Sciences·Maria SchindlerAnne Navarrete Santos
Feb 24, 2020·The Journal of Obstetrics and Gynaecology Research·Ayeh BoloukiSanaz Alaee
Sep 24, 2019·Endocrine, Metabolic & Immune Disorders Drug Targets·Ifeanacho Mercy OnuekwuzuIkewuchi Jude Chigozie
Apr 10, 2013·Endocrinology·Maria SchindlerAnne Navarrete Santos
Dec 6, 2016·Reproduction, Fertility, and Development·S Mareike PendzialekAnne Navarrete Santos
Feb 8, 2019·Endocrine, Metabolic & Immune Disorders Drug Targets·Ifeanacho Mercy OnuekwuzuIkewuchi Jude Chigozie
May 11, 2019·Endocrine Reviews·Andrew M KelleherThomas E Spencer
Sep 27, 2020·Molecular Human Reproduction·Maria SchindlerAnne Navarrete Santos
Oct 4, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Catherine Chidinma IkewuchiMercy Onuekwuzu Ifeanacho

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