Maternal hypercholesterolemia during pregnancy promotes early atherogenesis in LDL receptor-deficient mice and alters aortic gene expression determined by microarray

Circulation
C NapoliW Palinski

Abstract

Maternal hypercholesterolemia during pregnancy is associated with markedly enhanced fatty streak formation in human fetal aortas and accelerated progression of atherosclerosis in normocholesterolemic children. To establish the causal role of maternal hypercholesterolemia in a genetically homogeneous murine model and to test the hypothesis that pathogenic events during fetal development result in persistent changes in arterial gene expression, female LDL receptor-deficient (LDLR(-/-)) mice were fed regular chow or high-fat diets supplemented with 0.075% or 1.25% cholesterol during pregnancy. Lesion sizes were determined in the aortic origin of their chow-fed offspring at 3 months. Maternal hypercholesterolemia more than doubled lesion sizes in male offspring (P<0.0001 for the 0.0075% cholesterol group). Microarray analysis of the expression of 11 000 murine genes in the nonatherosclerotic descending aorta by Affymetrix gene chips suggested that 139 genes were significantly regulated in offspring of hypercholesterolemic mothers. A subset of 12 of the upregulated transcripts was subjected to secondary analysis by semiquantitative PCR of pooled RNA and 4 genes were found to be upregulated >1.7-fold. Quantitative PCR for one of thes...Continue Reading

References

Jul 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·A MontesJ J Albers
Dec 1, 1994·Nature Genetics·A J Villar, R A Pedersen
May 1, 1994·The Journal of Clinical Investigation·S IshibashiD K Burns
Dec 31, 1997·Science·R F BonnerL A Liotta
Apr 30, 1998·Current Opinion in Cell Biology·T Finkel
Nov 5, 1998·The Journal of Clinical Investigation·M Sata, K Walsh
Jan 23, 1999·Nature Genetics·D J DugganJ M Trent
Apr 21, 1999·Current Opinion in Cell Biology·F Mercurio, A M Manning
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·J KhanP S Meltzer
Jul 6, 2000·Trends in Cardiovascular Medicine·W Palinski, C Napoli
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·R O StuartS K Nigam

❮ Previous
Next ❯

Citations

Aug 6, 2009·Journal of Cardiovascular Translational Research·Wulf PalinskiClaudio Napoli
Apr 28, 2005·Free Radical Biology & Medicine·Scott W Ballinger
Sep 11, 2008·The British Journal of Nutrition·Zoe YatesAndrew M Salter
Jun 28, 2014·Journal of Developmental Origins of Health and Disease·H L Blackmore, S E Ozanne
Jun 24, 2011·Journal of Human Hypertension·M L de Gusmão CorreiaC A Mandarim-de-Lacerda
Oct 15, 2008·Nature Reviews. Genetics·Aldons J LusisKaren Reue
Dec 21, 2002·Asia Pacific Journal of Clinical Nutrition·John P NewnhamJohn R G Challis
Jan 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Filomena de NigrisClaudio Napoli
Feb 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Claudio NapoliPierGiuseppe Pelicci
Jul 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Klára KitajkaLászló G Puskás
Jun 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Claudio NapoliLouis J Ignarro
Mar 23, 2005·Proceedings of the National Academy of Sciences of the United States of America·Filomena de NigrisClaudio Napoli
Sep 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Toshio HayashiLouis J Ignarro
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Claudio NapoliLouis J Ignarro
Jun 19, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Piran ShelleyDenise S Fernandez-Twinn
May 3, 2011·PloS One·Valerio CostaAlfredo Ciccodicola
Feb 9, 2010·Journal of Women's Health·Reid F Thompson, Francine H Einstein
Mar 7, 2012·Expert Review of Cardiovascular Therapy·D Meeike KustersBarbara A Hutten
Dec 10, 2008·Endothelium : Journal of Endothelial Cell Research·Ines Martin-PaduraClaudio Napoli
Sep 24, 2005·Annals of Medicine·Tiina T TuomistoSeppo Ylä-Herttuala
Feb 3, 2016·Journal of Developmental Origins of Health and Disease·E J TarlingS C Langley-Evans
Nov 19, 2004·Neoplasia : an International Journal for Oncology Research·Andreas KurtzAnton Wellstein
Sep 10, 2010·International Journal of Cardiology·Genovefa D KolovouKatherine K Anagnostopoulou
Feb 24, 2009·Biochimica Et Biophysica Acta·Mikko P TurunenSeppo Ylä-Herttuala

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiology Journals

Discover the latest cardiology research in this collection of the top cardiology journals.

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.