Maternal LAMP/p55gagHIV-1 DNA immunization induces in utero priming and a long-lasting immune response in vaccinated neonates.

PloS One
Paula O RigatoMaria Notomi Sato

Abstract

Infants born to HIV-infected mothers are at high risk of becoming infected during gestation or the breastfeeding period. A search is thus warranted for vaccine formulations that will prevent mother-to-child HIV transmission. The LAMP/gag DNA chimeric vaccine encodes the HIV-1 p55gag fused to the lysosome-associated membrane protein-1 (LAMP-1) and has been shown to enhance anti-Gag antibody (Ab) and cellular immune responses in adult and neonatal mice; such a vaccine represents a new concept in antigen presentation. In this study, we evaluated the effect of LAMP/gag DNA immunization on neonates either before conception or during pregnancy. LAMP/gag immunization of BALB/c mice before conception by the intradermal route led to the transfer of anti-Gag IgG1 Ab through the placenta and via breastfeeding. Furthermore, there were an increased percentage of CD4+CD25+Foxp3+T cells in the spleens of neonates. When offspring were immunized with LAMP/gag DNA, the anti-Gag Ab response and the Gag-specific IFN-γ-secreting cells were decreased. Inhibition of anti-Gag Ab production and cellular responses were not observed six months after immunization, indicating that maternal immunization did not interfere with the long-lasting memory respons...Continue Reading

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Citations

Aug 3, 2012·Immunotherapy·Maria Notomi Sato
Apr 26, 2017·Human Vaccines & Immunotherapeutics·Fábio da Ressureição SgnottoJefferson Russo Victor
May 16, 2019·Archivum Immunologiae Et Therapiae Experimentalis·Ludimila Souza SantosJefferson Russo Victor

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Datasets Mentioned

BETA
KO3455
J03881

Methods Mentioned

BETA
ELISA
enzyme-
linked immunosorbent assay
FCS
flow cytometry

Software Mentioned

GraphPad Prism
ImmunoSpot

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