Maternal Torso-Like Coordinates Tissue Folding During Drosophila Gastrulation

Genetics
Travis K JohnsonCoral G Warr

Abstract

The rapid and orderly folding of epithelial tissue during developmental processes such as gastrulation requires the precise coordination of changes in cell shape. Here, we report that the perforin-like protein Torso-like (Tsl), the key extracellular determinant for Drosophila embryonic terminal patterning, also functions to control epithelial morphogenesis. We find that tsl null mutants display a ventral cuticular hole phenotype that is independent of the loss of terminal structures, and arises as a consequence of mesoderm invagination defects. We show that the holes are caused by uncoordinated constriction of ventral cell apices, resulting in the formation of an incomplete ventral furrow. Consistent with these data, we find that loss of tsl is sensitive to gene dosage of RhoGEF2, a critical mediator of Rho1-dependent ventral cell shape changes during furrow formation, suggesting that Tsl may act in this pathway. In addition, loss of tsl strongly suppressed the effects of ectopic expression of Folded Gastrulation (Fog), a secreted protein that promotes apical constriction. Taken together, our data suggest that Tsl controls Rho1-mediated apical constriction via Fog. Therefore, we propose that Tsl regulates extracellular Fog acti...Continue Reading

References

Apr 6, 1989·Nature·F SprengerC Nüsslein-Volhard
Oct 1, 1985·Developmental Biology·S B Zusman, E F Wieschaus
Oct 22, 1982·Science·G M Rubin, A C Spradling
Dec 1, 1993·Genes & Development·S Savant-Bhonsale, D J Montell
Jan 1, 1995·Annual Review of Cell and Developmental Biology·M Leptin
Nov 21, 1998·Current Biology : CB·E Knust, H J Müller
Dec 22, 1998·Mechanisms of Development·P Rørth
Dec 23, 1999·Current Biology : CB·C P Ponting
Mar 24, 2000·Science·G M RubinD A Harvey
Jul 9, 2002·Current Opinion in Genetics & Development·Y Tony Ip, Thomas Gridley
Jan 23, 2003·Genome Biology·Pavel TomancakGerald M Rubin
Aug 27, 2005·Development·Rachel E Dawes-HoangEric F Wieschaus
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Johannes BischofKonrad Basler
Jul 24, 2007·Nature Reviews. Molecular Cell Biology·Thomas Lecuit, Pierre-François Lenne
Aug 25, 2007·Science·Carlos J RosadoJames C Whisstock
Sep 16, 2009·Developmental Biology·Jacob M SawyerBob Goldstein
Mar 3, 2010·The Journal of Cell Biology·Adam C MartinEric F Wieschaus
Oct 25, 2012·Scientific Reports·Marco GrilloJordi Casanova
Feb 27, 2013·Developmental Biology·Elizabeth J DuncanPeter K Dearden
Aug 21, 2013·Proceedings of the National Academy of Sciences of the United States of America·Travis K JohnsonCoral G Warr
Nov 14, 2013·Science Signaling·Alyssa J ManningStephen L Rogers

❮ Previous
Next ❯

Citations

Apr 29, 2020·G3 : Genes - Genomes - Genetics·Daniel BakopoulosTravis K Johnson
Jun 19, 2019·Biochemical Society Transactions·Daniel BakopoulosTravis K Johnson
Jul 25, 2020·Current Biology : CB·Heath E JohnsonJared E Toettcher

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.