Maternally-Expressed Gene 3 (MEG3)/miR-143-3p Regulates Injury to Periodontal Ligament Cells by Mediating the AKT/Inhibitory κB Kinase (IKK) Pathway.

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Yao DongFeijun Dong

Abstract

BACKGROUND Dysregulated long noncoding RNAs (lncRNAs) are implicated in periodontitis development. Nevertheless, the role and mechanism of lncRNA maternally-expressed gene 3 (MEG3) in periodontitis progression remain unclear. This study aimed to explore how and whether MEG3 affect viability, apoptosis, and inflammatory response in lipopolysaccharide (LPS)-treated periodontal ligament cells (PDLCs). MATERIAL AND METHODS Periodontal ligament tissues were collected from periodontitis patients or normal individuals. PDLCs were obtained from normal periodontal ligament and treated with lipopolysaccharide (LPS). LPS-induced PDLCs injury was assessed via viability, apoptosis and inflammatory response using Cell Counting Kit-8, flow cytometry, quantitative reverse transcription polymerase chain reaction, enzyme-linked immunosorbent assay, and Western blot. The levels of MEG3 and microRNA (miR)-143-3p were examined via quantitative reverse transcription polymerase chain reaction. The protein kinase B(AKT)/inhibitory kappaB kinase (IKK) pathway was analyzed via Western blot. The target correlation of MEG3 and miR-143-3p was determined through dual-luciferase reporter analysis. RESULTS MEG3 level was decreased and miR-143-3p level was upr...Continue Reading

Citations

Feb 6, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shun ZhangWujun Chen
Jun 15, 2021·Seminars in Cell & Developmental Biology·Xianghong LuanThomas G H Diekwisch
Jul 24, 2021·Journal of Periodontal Research·Jingchen XuSong Chen

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Methods Mentioned

BETA
ELISA
transfection
flow cytometry

Software Mentioned

GraphPad Prism

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