Maternally transmitted milk containing recombinant human catalase provides protection against oxidation for mouse offspring during lactation

Free Radical Biology & Medicine
Zuyong HeNing Li

Abstract

Catalase plays an important role in protecting organisms against oxidative damage caused by reactive oxygen species (ROS) by degrading surplus hydrogen peroxide. Addition of exogenous catalase can alleviate injuries caused by ROS. Thus, production of human catalase through genetic engineering will meet the increasing therapeutic demand for this enzyme. In this study, we successfully expressed the recombinant gene in mouse mammary gland, and biologically active human catalase was secreted into the milk of the transgenic mice. The peroxisomal targeting sequence (PTS) within the catalase gene had no significant negative effect on the secretion of the recombinant protein. Intake of the transgenic milk by the pups was found to decrease lipid peroxidation, increase the total superoxide dismutase (T-SOD) activity in the brain, and enhance the total antioxidative capacity (T-AOC) of brain, liver, and serum. To our knowledge, this is the first example of efficient production of biologically active human catalase in the milk of transgenic animals. Our study suggests that scaled-up production in transgenic farm animals would yield sufficient human catalase for biomedical research and clinical therapies.

References

Jul 14, 1995·Mechanisms of Ageing and Development·J Q MoJ K Andersen
Apr 30, 1998·Physiological Reviews·K B Beckman, B N Ames
Oct 9, 1999·Free Radical Biology & Medicine·B XuP N Epstein
Feb 10, 2000·Acta Crystallographica. Section D, Biological Crystallography·T P KoD J Abraham
Jan 3, 2001·Acta Crystallographica. Section D, Biological Crystallography·M K SafoT P Ko
Nov 22, 2001·Journal of Neural Transmission·S LeutnerW E Müller
Sep 5, 2002·Free Radical Biology & Medicine·Rajindar S Sohal
Apr 10, 2003·Free Radical Biology & Medicine·Thomas D SweitzerVladimir R Muzykantov
Jul 1, 1956·Journal of Gerontology·D HARMAN
Aug 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Joshua B PlotkinArnold J Levine
May 10, 2005·Science·Samuel E SchrinerPeter S Rabinovitch
Jul 5, 2005·Expert Opinion on Investigational Drugs·K E Van Cott, W H Velander
Jul 5, 2005·Expert Opinion on Investigational Drugs·R G Werner
Sep 8, 2005·Rejuvenation Research·Richard G Cutler
Apr 29, 2006·The Journal of Biological Chemistry·Hamid Reza RezvaniHubert de Verneuil
Aug 19, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Kevin C Kregel, Hannah J Zhang
Dec 30, 2006·PLoS Genetics·Kimberly A DittmarTao Pan
Apr 6, 2007·Protein Expression and Purification·Xun-Long ShiPei Zhou

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Citations

Sep 28, 2014·Biotechnology Advances·Balwinder Singh SoochMunish Puri
May 29, 2015·Molecular Medicine Reports·Libin YangXuexin Chen
Jun 18, 2010·Meat Science·Cameron FaustmanSurendranath P Suman
Mar 15, 2015·Biological Reviews of the Cambridge Philosophical Society·Jonathan D BlountMichael A Cant
Aug 6, 2015·Food & Function·Xiao-Lin YuRui-Tian Liu
Mar 23, 2011·Toxicology Mechanisms and Methods·Lingfeng MinMingxuan Xie
Mar 17, 2010·Journal of Animal Science·S C FahrenkrugB P Glenn

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