Mathematical modeling and fluorescence imaging to study the Ca2+ turnover in skinned muscle fibers

Biophysical Journal
D UttenweilerRainer H A Fink

Abstract

A mathematical model was developed for the simulation of the spatial and temporal time course of Ca2+ ion movement in caffeine-induced calcium transients of chemically skinned muscle fiber preparations. Our model assumes cylindrical symmetry and quantifies the radial profile of Ca2+ ion concentration by solving the diffusion equations for Ca2+ ions and various mobile buffers, and the rate equations for Ca2+ buffering (mobile and immobile buffers) and for the release and reuptake of Ca2+ ions by the sarcoplasmic reticulum (SR), with a finite-difference algorithm. The results of the model are compared with caffeine-induced spatial Ca2+ transients obtained from saponin skinned murine fast-twitch fibers by fluorescence photometry and imaging measurements using the ratiometric dye Fura-2. The combination of mathematical modeling and digital image analysis provides a tool for the quantitative description of the total Ca2+ turnover and the different contributions of all interacting processes to the overall Ca2+ transient in skinned muscle fibers. It should thereby strongly improve the usage of skinned fibers as quantitative assay systems for many parameters of the SR and the contractile apparatus helping also to bridge the gap to the ...Continue Reading

References

Jan 1, 1977·Physiological Reviews·M Endo
Feb 1, 1977·Circulation Research·A Fabiato, F Fabiato
Jan 1, 1992·Progress in Biophysics and Molecular Biology·A F Dulhunty
Aug 1, 1987·Pflügers Archiv : European journal of physiology·R H Fink, D G Stephenson
Jan 1, 1988·Methods in Enzymology·M Endo, M Iino
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·D H MacLennan, P T Wong
Dec 1, 1982·Journal of Muscle Research and Cell Motility·J M GillisR H Kretsinger
Jan 15, 1994·The Journal of Physiology·G D LambD G Stephenson
May 8, 1995·Biochimica Et Biophysica Acta·W MelzerH C Lüttgau
Jul 1, 1994·Biophysical Journal·G J Kargacin
Jul 1, 1994·Biophysical Journal·J Wagner, J Keizer
Aug 1, 1995·The Journal of General Physiology·P C PapeW K Chandler
Jun 1, 1996·The Journal of Physiology·N Shirokova, E Ríos

❮ Previous
Next ❯

Citations

Jun 18, 2003·Pflügers Archiv : European journal of physiology·Aicha BouhlelClaude Léoty
Jul 10, 1999·The Journal of General Physiology·E RíosN Shirokova
Apr 25, 2008·Critical Care Medicine·Wolfgang ZinkBernhard M Graf
Sep 28, 2004·Journal of Biomedical Optics·Martin BothDietmar Uttenweiler
Dec 15, 2006·American Journal of Physiology. Cell Physiology·Gennadi M KravtsovTak Ming Wong

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.