Mathematical modeling of positron emission tomography (PET) data to assess radiofluoride transport in living plants following petiolar administration

Plant Methods
Alexander K ConverseOnofre T DeJesus

Abstract

Ion transport is a fundamental physiological process that can be studied non-invasively in living plants with radiotracer imaging methods. Fluoride is a known phytotoxic pollutant and understanding its transport in plants after leaf absorption is of interest to those in agricultural areas near industrial sources of airborne fluoride. Here we report the novel use of a commercial, high-resolution, animal positron emission tomography (PET) scanner to trace a bolus of [(18)F]fluoride administered via bisected petioles of Brassica oleracea, an established model species, to simulate whole plant uptake of atmospheric fluoride. This methodology allows for the first time mathematical compartmental modeling of fluoride transport in the living plant. Radiotracer kinetics in the stem were described with a single-parameter free- and trapped-compartment model and mean arrival times at different stem positions were calculated from the free-compartment time-activity curves. After initiation of administration at the bisected leaf stalk, [(18)F] radioactivity climbed for approximately 10 minutes followed by rapid washout from the stem and equilibration within leaves. Kinetic modeling of transport in the stem yielded a trapping rate of 1.5 +/- 0....Continue Reading

References

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Citations

Oct 7, 2015·Trends in Plant Science·Michiel Hubeau, Kathy Steppe
Mar 14, 2019·Journal of Fish Biology·Arthur N Popper, Anthony D Hawkins
Mar 4, 2020·Journal of the Science of Food and Agriculture·Hui-Liang NiuHui-Mei Cai
Jun 23, 2018·Frontiers in Plant Science·Jonas BühlerGregor J Huber
Jan 21, 2021·Plant Methods·Gihan P RuwanpathiranaHerbert J Kronzucker

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Methods Mentioned

BETA
imaging technique

Software Mentioned

ASIPro
Siemens ASIPro VM
microPET Manager
Siemens

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