Mathematical models for CFSE labelled lymphocyte dynamics: asymmetry and time-lag in division

Journal of Mathematical Biology
Tatyana LuzyaninaGennady Bocharov

Abstract

Since their invention in 1994, fluorescent dyes such as carboxyfluorescein diacetate succinimidyl ester (CFSE) are used for cell proliferation analysis in flow cytometry. Importantly, the interpretation of such assays relies on the assumption that the label is divided equally between the daughter cells upon cell division. However, recent experimental studies indicate that division of cells is not perfectly symmetric and there is unequal distribution of protein between sister cell pairs. The uneven partition of protein or mass to daughter cells can lead to an overlap in the generations of CFSE-labelled cells with straightforward consequences for the resolution of individual generations. Numerous mathematical models developed so far for the analysis of CFSE proliferation assay incorporate the premise that the CFSE fluorescence intensity is halved in the two daughter cells. Here, we propose a novel modelling approach for the analysis of the CFSE cell proliferation assays which are characterized by poorly resolved peaks of cell generations in flow cytometric histograms. We formulate a mathematical model in the form of a system of delay hyperbolic partial differential equations which provides a good agreement with the CFSE histogram...Continue Reading

References

Jun 1, 1977·The British Journal of Venereal Diseases·J B Robbins
Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J A Smith, L Martin
Jan 1, 1994·Journal of Mathematical Biology·M C Mackey, R Rudnicki
May 2, 1994·Journal of Immunological Methods·A B Lyons, C R Parish
Nov 26, 1999·Immunology and Cell Biology·R E NordonR Odell
Oct 25, 2003·Journal of Theoretical Biology·Sergei S PilyuginRustom Antia
Aug 13, 2004·European Journal of Immunology·Burkhard LudewigGennady Bocharov
Nov 13, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Giada MateraPaolo Ubezio
Apr 26, 2005·Journal of Immunological Methods·Vitaly V GanusovRustom Antia
Jan 18, 2006·Nature Reviews. Microbiology·Cornelia C BergmannStephen A Stohlman
Feb 21, 2006·Journal of Theoretical Biology·Nikos V Mantzaris
Nov 10, 2006·Journal of Mathematical Biology·Tatyana LuzyaninaGennady Bocharov
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·E D HawkinsP D Hodgkin
Jul 26, 2007·Theoretical Biology & Medical Modelling·Tatyana LuzyaninaGennady Bocharov
Jul 27, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Kap-Hyoun KoRobert E Nordon
Jul 29, 2008·Journal of Pediatric Endocrinology & Metabolism : JPEM·Robert RapaportUNKNOWN Pfizer SGA Collaborative Study Group
Sep 13, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Paul K WallaceKatharine A Muirhead
Apr 22, 2009·Bulletin of Mathematical Biology·Ha Youn LeeAlan S Perelson
Mar 3, 2010·Bulletin of Mathematical Biology·H T BanksAndreas Meyerhans
Jan 26, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Mario Roederer
Feb 22, 2011·Journal of Theoretical Biology·Robert E NordonTimm Schroeder
May 5, 2011·Biotechnology Advances·R Lencastre FernandesK V Gernaey
Jun 18, 2011·Bulletin of Mathematical Biology·Hongyu MiaoHulin Wu
Sep 6, 2011·Journal of Immunological Methods·H T BanksAndreas Meyerhans
Mar 1, 2012·Journal of Immunological Methods·Benjamin J C Quah, Christopher R Parish
Mar 13, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Vahe AkbarianJulie Audet
Apr 3, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria L CioccaSteven L Reiner
Jan 15, 2013·Mathematical Biosciences and Engineering : MBE·H T BanksAndreas Meyerhans
Jan 15, 2013·Journal of Theoretical Biology·Rob J De Boer, Alan S Perelson
Mar 26, 2013·Applied Mathematics Letters·H T BanksW C Thompson
Jul 6, 2013·Journal of Biological Dynamics·H T BanksAndreas Meyerhans

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Citations

Feb 13, 2019·Bioinformatics·Marco S NobileDaniela Besozzi
Nov 7, 2019·PLoS Computational Biology·Valerya ZheltkovaAndreas Meyerhans
Mar 3, 2020·Frontiers in Cellular and Infection Microbiology·Bruno Carvalho ResendeCarlos Renato Machado

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