Mating changes the subcellular distribution and the functionality of estrogen receptors in the rat oviduct.

Reproductive Biology and Endocrinology : RB&E
Pedro A OrihuelaH B Croxatto

Abstract

Mating changes the mode of action of 17beta-estradiol (E2) to accelerate oviductal egg transport from a nongenomic to a genomic mode, although in both pathways estrogen receptors (ER) are required. This change was designated as intracellular path shifting (IPS). Herein, we examined the subcellular distribution of ESR1 and ESR2 (formerly known as ER-alpha and ER-beta) in oviductal epithelial cells of rats on day 1 of cycle (C1) or pregnancy (P1) using immunoelectron microscopy for ESR1 and ESR2. The effect of mating on intraoviductal ESR1 or ESR2 signaling was then explored comparing the expression of E2-target genes c-fos, brain creatine kinase (Ckb) and calbindin 9 kDa (s100g) in rats on C1 or P1 treated with selective agonists for ESR1 (PPT) or ESR2 (DPN). The effect of ER agonists on egg transport was also evaluated on C1 or P1 rats. Receptor immunoreactivity was associated with the nucleus, cytoplasm and plasma membrane of the epithelial cells. Mating affected the subcellular distribution of both receptors as well as the response to E2. In C1 and P1 rats, PPT increased Ckb while both agonists increased c-fos. DPN increased Ckb and s100g only in C1 and P1 rats, respectively. PPT accelerated egg transport in both groups and D...Continue Reading

References

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Citations

Nov 14, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·María L OrósticaPedro A Orihuela
Nov 2, 2014·Molecular Reproduction and Development·María L OrósticaPedro A Orihuela
Jul 15, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Patricia ReuquénPedro A Orihuela
Oct 26, 2016·Molecular Reproduction and Development·Alexis Parada-BustamantePedro A Orihuela
Jul 21, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·María L OrósticaPedro A Orihuela
Mar 27, 2018·General and Comparative Endocrinology·Mariana Machado-NevesCleida Aparecida de Oliveira

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