PMID: 7007817Jan 1, 1980Paper

Mating due to loss of surface exclusion as a cause for thermosensitive growth of bacteria containing the Rtsl plasmid

Molecular & General Genetics : MGG
J T Ou

Abstract

At 25 degrees C, Rtsl+ bacteria grow to about 5 X 10(9) bacterial/ml before leveling off, whereas at 42 degrees C they grow from 2.6 X 10(8) bacteria/ml for only 2-3 generations after temperature shift before the growth is inhibited with a zig-zag pattern at the plateau. When diluted, Rtsl+ bacteria grow rapidly at 42 degrees C, until the concentration reaches as high as the undiluted 42 degrees C culture when growth measured by colony counts stops and the zig-zag pattern again appears. This density-dependent growth inhibition is not due to the presence of stable growth inhibitor(s). Mating experiments show that at 42 degrees C, Rtsl+ bacteria retain good donor ability; at the same time, they become good recipients in mating with Hfr (Rtsl) bacteria. SDS-PAGE reveals that membranes are altered at 42 degrees C. Examination of DNA synthesis indicates that chromosomal DNA is synthesized at both 25 degrees C and 42 degrees C at high bacterial concentration, but that of the Rtsl plasmid is slowed down at 42 degrees C. The labeling experiments suggest that in 2 h there are 2 rounds of plasmid replication at 25 degrees C, 3.5 rounds at 42 degrees C when bacteria are diluted, and 0.6 rounds at 42 degrees C when bacteria are not diluted...Continue Reading

References

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