Nov 2, 1976

Mating reaction in yeast protoplasts

Archives of Microbiology
A Svoboda

Abstract

Protoplasts prepared from complementary haploid strains of Saccharomyces cervisiae were studied with regard to their ability of conjugating. Neither fresh protoplasts nor the growing protoplasts possessing fibrillar walls exhibited sex specific agglutination or fusion. However, they were capable of inducing sexual activation in normal cells of opposite mating type. After completing the regeneration of cell walls the protoplasts could conjugate either with each other or with cells of opposite sex. The frequency of conjugations was low, about 1%, and was largely dependent on the degree of completition of the wall during regeneration. From the results the following conclusions may be drawn: 1. The initiation of mating is dependent on the integrity of the cell wall. 2. The sex specific morphogenetic changes do not occur in wall-less protoplasts but may happen after the protoplasts have regenerated their cell walls. 3. The lysis of cell walls does not occur until the walls come into close contact. 4. The fusion of plasma membranes in sex-activated protoplasts cannot be induced by arteficial agglutination.

  • References10
  • Citations2

References

  • References10
  • Citations2

Citations

Mentioned in this Paper

Pathologic Cytolysis
Transcription Initiation
Protoplasts
Saccharomyces
Cell Wall
Observation Method - Agglutination
Natural Regeneration
Agglutination
Saccharomyces cerevisiae
Bacterial Conjugation

About this Paper

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.

© 2020 Meta ULC. All rights reserved