Mating rhythms of Drosophila: rescue of tim01 mutants by D. ananassae timeless

Journal of Circadian Rhythms
Izumi NishinokubiNorio Ishida

Abstract

It is reported that the circadian rhythms of female mating activity differ among Drosophila species and are controlled by an endogenous circadian clock. Here, we found that the mating rhythm of D. ananassae differed from that of D. melanogaster. Moreover, to evaluate the effect of clock gene products on mating activities, we examined the mating activity of D. melanogaster timeless (tim01) transgenic fly harboring heat-shock promotor driven-D. ananassae timeless (tim) gene (hs-AT tim01). Flies were maintained under light/dark (LD) cycles for several days and then they were transferred to constant dark (DD) conditions at 25 degrees C. Transformant flies were heat-shocked for 30 min (PZT 10.5-11.0 or PZT 22.5-23.0; PZT means Projected Zeitgeber Time) at 37 degrees C every day. Daily expressions of D. ananassae TIMELESS (TIM) protein in transgenic flies were measured by western blotting. To examine whether the timing of D. ananassae TIM protein induction by heat shock can change the patterns of the behavior activities of D. melanogaster tim01 flies, we measured locomotor and mating activity rhythms under DD at 25 degrees C +/- 0.5 degrees C except when heat shock was applied. Heat shock applied at PZT 10.5-11.0 and at PZT 22.5-23.0...Continue Reading

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Citations

Jan 5, 2012·Journal of Biological Rhythms·Esteban J BeckwithHoracio O de la Iglesia
Oct 1, 2018·The European Journal of Neuroscience·Charlotte Helfrich-FörsterPamela Menegazzi
Nov 19, 2010·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yasutaka HamasakaNorio Ishida
Jun 28, 2012·The Journal of Comparative Neurology·Christiane HermannCharlotte Helfrich-Förster

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Methods Mentioned

BETA
transgenic

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